摘要: It is well known that voltage-gated calcium channels (VGCCs)-mediated Ca2+ influx triggers evoked synaptic vesicle release. However, the mechanisms of Ca2+ regulation of spontaneous miniature vesicle release (mini) remain poorly understood. Here we show that blocking VGCCs at the juvenile mice (C57BL/6) calyx of Held synapse failed to cause an immediate change in minis. Instead, it resulted in a significant reduction (similar to 40%) of mini frequency several minutes after the blockage. By recording VGCC activity and single vesicle fusion events directly at the presynaptic terminal, we found that minis did not couple to VGCC-mediated Ca2+ entry, arguing for a lack of direct correlation between mini and transient Ca2+ influx. Moreover, mini frequencies displayed a lower apparent Ca2+ cooperativity than those of evoked release. In agreement with this observation, abrogation of the Ca2+ sensor synaptotagmin-2 had no effect on apparent Ca2+ cooperativity of minis. Together, our study provides the first direct evidence that spontaneous minis are not mediated by transient Ca2+ signals through VGCCs and are triggered by a Ca2+-sensing mechanism that is different from the evoked release at these microdomain VGCC-vesicle coupled synapses.
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期刊:
JOURNAL OF NEUROSCIENCE
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分类:
生物学
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生物物理学
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神经科学
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引用:
ChinaXiv:201605.01333
(或此版本
ChinaXiv:201605.01333V1)
doi:10.12074/201605.01333
CSTR:32003.36.ChinaXiv.201605.01333.V1
- 推荐引用方式:
Dai, Jinye,Chen, Peihua,Tian, Hao,Sun, Jianyuan,Dai, Jinye,Tian, Hao,Sun, Jianyuan,Chen, Peihua,Sun, Jianyuan.(2016).Spontaneous Vesicle Release Is Not Tightly Coupled to Voltage-Gated Calcium Channel-Mediated Ca2+ Influx and Is Triggered by a Ca2+ Sensor Other Than Synaptotagmin-2 at the Juvenile Mice Calyx of....JOURNAL OF NEUROSCIENCE.[ChinaXiv:201605.01333]
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