您当前的位置: > 详细浏览

microRNA-107在肝癌中的表达及临床意义

摘要: 目的 检测microRNA-107(miR-107)在肝癌中的表达及意义,并探讨其对肝癌细胞增殖能力的影响。方法 利用公共基因表达数据库(TCGA数据库和GEO数据库)中的肝癌microRNA表达谱数据库,分析miR-107在肝癌及癌旁的表达水平;收集22例肝癌及其相应癌旁新鲜组织标本,qRT-PCR方法检测miR-107的表达水平;收集53例石蜡包埋的肝癌组织标本,qRT-PCR方法检测miR-107表达水平并分析其在肝癌中的临床意义。在人肝癌细胞株Huh7中过表达或沉默miR-107,通过MTT方法检测其对肝癌细胞增殖能力的影响。结果 miR-107在肝癌组织中的表达显著高于其在癌旁组织中的表达,差异具有统计学意义(P<0.05)。在肝癌组织中,miR-107与肿瘤大小呈正相关关系(P=0.032)。体外实验结果表明,过表达miR-107后肝癌细胞增殖能力明显增强(P<0.0001),而沉默miR-107后细胞增殖能力明显降低(P<0.0001)。结论 miR-107在肝癌中表达上调,其高表达与肝癌细胞增殖密切相关,miR-107可能是一个与肝癌的发生发展相关的基因。

Abstract: Objective To investigate the expression of microRNA-107 (miR-107) and its functional role in hepatocellular carcinoma (HCC). Methods The gene chip data of HCC obtained from the Gene Expression Omnibus (GEO) database and the Cancer Genome Atlas (TCGA) database were used to analyze the expression levels of miR-107 in liver cancer. Twenty-two pairs of fresh surgical specimens of HCC and adjacent tissues and 53 paraffin-embedded specimens of HCC were examined for miR-107 expression by qRT-PCR. The correlation of the expression levels of miR-107 with the clinicopathologic characteristics of the patients were analyzed. The role of miR-107 in regulating the proliferation of hepatocellular carcinoma cells were determined by MTT assay in Huh7 cells transfected with a miR-107 mimic or inhibitor. Results The expression levels of miR-107 were significantly up-regulated in HCC tissues as compared to the adjacent tissues (P<0.05) in positive correlation with the tumor size (P<0.032). Transfection with miR-107 mimics significantly promoted the cell proliferation (P< 0.0001) while miR-107 inhibitor inhibited the cell proliferation (P<0.0001). Conclusion The expression of miR-107 is upregulated in HCC tissues and its expression levels are correlated with HCC cell proliferation, suggesting its role as a potential oncogene in liver cancer.

版本历史

[V1] 2017-12-27 12:40:28 ChinaXiv:201712.00912V1 下载全文
点击下载全文
预览
许可声明
metrics指标
  •  点击量1181
  •  下载量622
评论
分享
邀请专家评阅