广西人群中白介素-33基因rs1891385A/C、rs10975519C/T 多态性的分布特点

• 作者： 郭静 ¹ 向阳 ² 黄华佗 ² 彭友帆 ² 韦叶生 ² 蓝艳 ¹
• 作者单位：
  1.  右江民族医学院附属医院 皮肤科，检验科，广西 百色 533000
• 提交时间：2017-12-27

摘要: 目的 研究探讨IL-33基因rs1891385A/C、rs10975519C/T两位点的多态性在广西壮、汉族人群中的分布特点。分析各基因型及等位基因的分布频率在广西人群中种族、性别间的差异。方法 采用单碱基延伸法（PCR-SEB）及DNA测序对283例受试者的 IL-33基因 rs1891385A/C、rs10975519C/T位点进行多态性分析。用统计学方法分析其分布频率及组间差异。结果rs1891385A/C 存在AA、AC、CC3种基因型，分布频率分别为64.3% 、32.5%、3.2% 。其各基因型及等位基因频率在广西人群中壮、汉，男、女性别之间无显著差异（P均>0.05）。其基因型与等位基因频率与人类基因组计划公布的欧洲人、非洲黑人之间均有显著差异（P0.05）。其基因型仅与欧洲人、日本人差异有统计学意义（P<0.01）。其等位基因频率仅与欧洲人群相比差异有统计学意义（P<0.01）。结论 IL-33基因rs1891385A/C、rs10975519C/T的基因多态性存在着不同程度的种族和地区差异。

Abstract: Objective To explore the race- and gender-specific distribution characteristics of rs1891385A/C and rs10975519C/T polymorphism of interleukin-33 (IL-33) gene in Zhuang and Han populations. Methods The polymorphisms of rs1891385A/C and rs10975519C/T of IL-33 gene in 283 subjects from Guangxi Zhuang Autonomous Region were analyzed with single base extension (PCR-SEB) and DNA sequencing to analyze the differences in their distribution frequencies between genders and between Zhuang and Han populations. Results Three genotypes (AA, AC and CC) were found in rs1891385A/C with frequencies of 64.3% , 32.5% and 3.2% , respectively. The genotype and allele frequencies of rs1891385A/C in this Guangxi population showed no significant difference between Zhuang and Han subpopulations and between genders (P>0.05), but differed significantly from those in European and African black populations (P0.05). The genotype frequency of rs10975519C/T in this population differed significantly from those in the European and Japanese populations (P<0.01), but the allele frequencies only showed significant differences from those in the European population (P<0.01). Conclusion rs1891385A/C and rs10975519C/T polymorphisms of IL-33 gene show a race-specific difference.

• 分类： 医学、药学 >> 基础医学
• 引用： ChinaXiv:201712.00914 (或此版本 ChinaXiv:201712.00914V1)
  doi:10.12074/201712.00914
  CSTR:32003.36.ChinaXiv.201712.00914.V1
• 推荐引用方式： 郭静,向阳,黄华佗,彭友帆,韦叶生,蓝艳.(2017).广西人群中白介素-33基因rs1891385A/C、rs10975519C/T 多态性的分布特点.南方医科大学学报.[ChinaXiv:201712.00914] (点此复制)