新型含锶硫酸钙的制备及其生物相容性试验

• 作者： 侯毅龙 ¹ 李雪 ¹ 许长鹏 ¹ 宋锦旗 ¹ 黄磊 ¹ 余斌 ¹
• 作者单位：
  1.  南方医科大学南方医院创伤骨科，广东 广州 510515
• 提交时间：2017-12-27

摘要: 目的 制备一种新型含锶硫酸钙材料并对其进行生物相容性评价。方法 探讨采用水热法制备一种新型的含锶硫酸钙并对制备的材料进行：（1）X线衍射、傅里叶变换红外光谱及差式量热扫描表征检测，以验证材料为目标产物；（2）细胞毒性试验评估材料浸提液对L-929小鼠成纤维细胞形态学及相对增殖率的影响；(3)溶血试验评估材料浸提液的血液相容性；（4）体内植入试验评估其对活体组织局部毒性反应。结果（1）X线衍射结果示:14.63°，25.72°和 29.80°处可见α-半水硫酸钙的特征峰，在24.78°处可见锶元素的特征峰;傅里叶变换红外光谱与α-半水硫酸钙标准图谱相似；差式量热扫描结果示：制备材料中结构水含量为6.03%；（2）细胞毒性试验显示被试材料浸提液在不同时间点的毒性反应均为I级，无细胞毒性；（3）溶血试验结果显示溶血率为4.3%，符合溶血试验要求；（4）体内植入结果表明材料和机体达到稳定状态。结论 该材料为含锶硫酸钙，生物相容性试验表明其具有良好的生物相容性。

Abstract: Objective To prepare a novel strontium-containing calcium sulfate and assess its and biocompatibility. Methods A novel strontium-containing α-calcium sulfate hemihydrate (Sr-caS) bone substitute as prepared with hydrothermal reaction and examined for X-ray diffraction (XRD), Fourier transform infrared (FTIR) and thermogravimetric differential scanning calorimetric (TG-DSC) patterns. The biocompatibility of the material was evaluated by in vitro cytotoxicity test in L-929 cells, hemolysis test of blood, and in vivo implantation test in SD rats. Results The XRD spectra of the prepared Sr-CaS powder highlighted 3 strong characteristic peaks of α-CaSO4 at 14.63°, 25.72° and 29.80° with a strontium-specific peak at 24.78°. The FTIR patterns of Sr-CaS resembled those of CaS. TG-DSC results showed that the material contained a non-evaporable water content of 6.03%. In vitro cytotoxicity test in L-929 cells suggested that the material had a class 1 cytotoxicity, and the hemolysis rate of its aqueous extract was 4.3%. The material implanted in the muscular tissues of SD rats maintained a steady state in the surrounding tissues. Conclusion This strontium-containing calcium sulfate material we prepared shows an excellent biocompatibility for potential use as a novel artificial bone material.

• 分类： 医学、药学 >> 基础医学
• 引用： ChinaXiv:201712.00917 (或此版本 ChinaXiv:201712.00917V1)
  doi:10.12074/201712.00917
  CSTR:32003.36.ChinaXiv.201712.00917.V1
• 推荐引用方式： 侯毅龙,李雪,许长鹏,宋锦旗,黄磊,余斌.(2017).新型含锶硫酸钙的制备及其生物相容性试验.南方医科大学学报.[ChinaXiv:201712.00917] (点此复制)