您当前的位置: > 详细浏览

胡蜂毒对类风湿关节炎实验模型具有潜在的治疗作用

Wasp Venom Possesses Potential Therapeutic Effect in Experimental Models of Rheumatoid Arthritis.

摘要:类风湿关节炎(RA)是一种自身免疫性疾病。胡蜂毒被认为是云南景颇族的一种传统民间药物,具有治疗类风湿性关节炎的作用。本研究旨在探讨胡蜂毒对实验性大鼠类风湿性关节炎的改善作用。我们建立了II型胶原(CII)诱导的SD大鼠关节炎(CIA)模型,并检测了炎症抑制作用和自身免疫反应。通过对患肢的肿胀、组织病理学评分及组织病理学改变来评价胡蜂毒(WV)的抗关节炎作用。检测了IL-6、TNF-α和IL-1β的表达水平以及外周血中炎症细胞的数量来。采用流式细胞术检测大鼠脾脏T细胞亚群比例的变化,同时测定脏器指数和免疫血清球蛋白水平。结果表明,不同剂量的WV(0.125、0.25、0.5 mg/kg)对CIA大鼠足肿胀和关节炎评分有显著的缓解作用(P < 0.05)。WV(0.25、0.5 mg/kg)能减轻大鼠踝关节滑膜组织病变,滑膜细胞增生、炎性细胞浸润的组织病理学评分(P < 0.05)。在免疫调节方面,0.5 mg/kg WV可降低免疫血清球蛋白水平(P < 0.05),我们进一步发现,0.5 mg/kg的WV可抑制Th细胞的免疫应答,同时显著增强脾脏细胞中Tc细胞和Treg细胞的功能(P < 0.05)。WV的免疫抑制作用类似于其对大鼠血清中IL-1β、TNF-α、IL-8、IL-6、cox-2和PGE2水平的抑制。综上所述,这些发现表明WV具有抗关节炎的活性,这可能与它们对免疫的调节和抗炎作用有关。

英文摘要:Rheumatoid arthritis (RA) is an autoimmune disease. Wasp venom (WV) relieves rheumatoid arthritis considered as a traditional folk medicine in Jingpo nationality in Yunnan, China. The current study aimed to investigate the effect of wasp venom ameliorating rheumatoid arthritis symptoms in experimental rats. We established a model of type II collagen (CII)-induced arthritis (CIA) in SD rats and examined the inhibition of inflammation and autoimmune response. The anti-arthritic effects of WV were evaluated through the paw swelling, histopathological score and histopathology changes of the affected paw were assessed. The anti-inflammation effects were assayed by the level of IL-6, TNF-α, IL-1β and the number of inflammatory cells in peripheral blood. The alteration of the T cell subsets ratio in the spleen of rats was detected by ?ow cytometry, at the same time the viscera index and immune serum globulin levels were evaluated. The results suggested that various doses of WV (0.125, 0.25 and 0.5 mg/kg) significantly alleviated paw swelling and arthritis score in CIA rats with the untreated control (P < 0.05). WV (0.25 and 0.5 mg/kg) relieved synovial tissue lesions of ankle joints and histopathology scores of synoviocyte hyperplasia and inflammatory cell infiltration with vehicle group (P < 0.05). Regarding immunological regulation, 0.5 mg/kg WV lowered the immune serum globulin levels (P < 0.05), and we further found that WV (0.5 mg/kg) suppressed the immune response of Th cells, while enhanced the functions of Tc cells and Treg cells in spleen cells markedly (P < 0.05). The immunosuppressive action of WV displayed was analogous to its inhibitory effect on IL-1β, TNF-α, IL-8, IL-6, COX-2 and PGE2 levels in rat serum. In conclusion, these findings demonstrated that WV exhibited anti-arthritic activity, which might be associated with their inhibitory effects on immunoregulation and anti-inflammatory action."

版本历史

[V2] 2020-05-05 18:54:44 chinaXiv:202003.00053V2 下载全文
[V1] 2020-03-13 23:53:00 chinaXiv:202003.00053v1 查看此版本 下载全文
点击下载全文
同行评议状态
待评议
许可声明
metrics指标
  • 点击量41057
  • 下载量3016
评论
分享
邀请专家评阅