To explore the changes of the urinary proteome in DEN induced liver cancer-inflammatory cancer model in rats

Abstract: [Objective] Through the liver cancer-inflammatory cancer model induced by DEN, observe the dynamic change process of urine proteome at different stages of the disease. [Methods] In this study, a rat liver cancer-inflammatory cancer model was constructed by intraperitoneal injection of DEN, urine was collected weekly, and differential proteins were identified by liquid chromatography coupled with mass spectrometry (LC-MS/MS), and the biological pathways of differential proteins were analyzed using IPA software. To observe the changes in urine protein at different stages of the disease in the rat liver cancer-inflammatory cancer model. [Results] Fifteen experimental rats were induced by DEN to construct different stages of the hepatitis-cirrhosis-liver cancer disease model, and each rat had a different disease progression due to individual differences. Each rat has more differential proteins at different disease time points. The differential proteins of different rats are enriched in the same biological pathways related to liver injury, inflammatory lesions, tumor occurrence, and potential therapeutic targets of the tumor. [Discussion] We can observe different changes of urine proteome in each stage of the liver cancer inflammatory cancer model, and different rats will have different disease progression due to individual differences, which suggests that we need to pay more attention to the individualization and accuracy of detection in the future. "