您当前的位置: > 详细浏览

外源褪黑素对小麦幼苗生理及光合荧光特性的影响

摘要:为探讨不同浓度外源褪黑素对小麦幼苗生理及光合荧光特性的影响,以良星 99 为供 试材料,测定了不同浓度褪黑素处理下小麦幼苗生长形态、光合及荧光参数以及抗氧化酶活 性等关键指标。结果表明:(1)0.1 μmol·L-1 的褪黑素显著提高了小麦植株的光合能力,叶 绿素 Chl a、Chl b 和 Chl (a+b)以及叶绿素荧光参数调节性能量耗散的量子产额 Y (NPQ)、表 观光合传递速率(ETR)和非光化学淬灭(NPQ)均在褪黑素浓度为 0.1 μmol·L-1 时达到增 加最大值;PSⅡ最大光合效率(Fv/Fm)、最大光能转化潜力(Fv/Fo)随褪黑素浓度升高 逐渐降低;光化学淬灭(qL)随褪黑素浓度增加先下降后上升。(2)与 CK(0 μmol·L-1) 相比低浓度褪黑素显著降低小麦根和叶中过氧化物酶(POD)及小麦叶中过氧化氢酶(CAT) 的活性,高浓度褪黑素显著增加了小麦 POD 的活性;小麦根中丙二醛(MDA)含量随褪黑 素浓度的增加先下降后上升。研究表明适量褪黑素处理可促进小麦的生长,使小麦光合能力 维持在较高水平,并通过 POD 和 CAT 调节不同褪黑素处理引起的氧化应激。该研究为进一 步深入了解褪黑素在小麦中的调控作用机制奠定了基础。

英文摘要:To explore the effects of different concentrations of melatonin on physiological and photosynthetic fluorescence characteristics of wheat seedlings. The growth morphology, photosynthetic, fluorescence parameters and antioxidant enzyme activity of wheat seedings treated with different concentrations of melatonin were determined by using Liangxing 99 as experimental material. The results were as follows: (1) 0.1 μmol·L-1 melatonin significantly increased the photosynthetic capacity of wheat plants, Chlorophyll a (Chl a), Chlorophyll b (Chl b) and Chlorophyll (a+b) [Chl (a+b)] increased to the maximum value when melatonin concentration was 0.1 μmol·L-1. Chlorophyll fluorescence parameters include parameters of quantum yield of regulated energy dissipation Y (NPQ), the electron transport rate (ETR) and non photochemical quenching (NPQ) also reaches the maximum at 0.1 μmol·L-1. The PSⅡ maximum photosynthetic efficiency (Fv/Fm) and maximum photochemical conversion potential (Fv/Fo) decreased with the increase of melatonin concentratio. Photochemical quenching (qL) decreased first and then increased with the increase of melatonin. (2) Compared with CK (0 μmol·L-1), low melatonin concentration significantly decreased peroxidase (POD) activity in wheat and decreased catalase (CAT) activity in wheat leaves, high melatonin concentration significantly increased POD activity in wheat. Low concentration of melatonin treatment significantly reduced the catalase (CAT) activity in wheat leaves by compared with CK. The content of malondialdehyde (MDA) in wheat roots decreased firstly and then increased with the increase of melatonin concentration. In conclusion, it suggested that appropriate melatonin treatment can promote the growth of wheat and maintain the photosynthetic capacity of wheat at a high level. Enzymes including POD and CAT can also regulate oxidative stress induced by different concentrations of melatonin in wheat seedlings. This study laid a foundation for further understanding the regulatory mechanism of melatonin in wheat.

版本历史

[V1] 2022-06-07 10:45:19 chinaXiv:202206.00098V1 下载全文
点击下载全文
许可声明
metrics指标
  • 点击量902
  • 下载量241
评论
分享
邀请专家评阅