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大粒香水稻叶绿体基因组特征分析

摘要:大粒香作为贵州重要的优质稻资源,推广种植面积大,且在乡村振兴过程中为社会带 来了较高的经济效益,但目前对大粒香基因组学理论研究报道不多。为了揭示大粒香水稻叶 绿体基因组特征及系统发育关系,该文对大粒香叶绿体进行测序,分析其基因组特征。结果 表明:(1)大粒香水稻 cpDNA 全长 134 563 bp,包括一个 LSC(80 864 bp),一个 SSC (12 347 bp)和两个 IRs(20 676 bp)。(2)注释到 129 个基因,可分为蛋白编码、tRNA 和 rRNA 三类基因,数量分别为 85 个、36 个和 8 个。(3)密码子偏好分析显示,大粒香 cpDNA 密码子偏好 A 碱基或者 U(T)碱基,亮氨酸密码子使用了 1 944 次,最多;半胱氨 酸的密码子仅使用 198 次,最少。(4)检测到 129 个 SSR,其中有 95 个单核苷酸重复,且 大部分 SSR 序列由 A/T 碱基组成。(5)系统发育分析结果表明,大粒香与热带粳稻亲缘关 系最近聚为一类。该研究揭示了大粒香叶绿体基因组信息,并确定了大粒香系统发育所属分 支。

英文摘要:As an important high-quality rice resource in Guizhou province, Dalixiang has a large planting area, and has brought higher economic benefits to society in the process of rural revitalization. However, there are few theoretical researches on the genomics of Dalixiang. In order to reveal the characteristics and phylogenetic relationships of chloroplast genome of large grain perfume rice, the chloroplasts of grancas fragrances were sequenced and their genomic characteristics were analyzed. The results were as follows: (1) The chloroplast genome of Dalixiang was 134 563 bp, including LSC(80 864 bp),SSC(12 347 bp)and two IRs(20 676 bp). (2) There were 129 genes annotated in the chloroplast genome of Dalixiang, which could be divided into protein coding, tRNA and rRNA, with 85, 36 and 8 genes respectively. (3) Codon bias analysis of Dalixiang showed that leucine was most frequently used and that cysteine was used least frequently, and most codons ended in A/U(T). (4) The total number of SSR loci in the cpDNA of Dalixiang was 129, ninety-five of which are mononucleotide and most of SSR was composed of nucleobase A/T. (5) Phylogenetic analysis shows an affiliation relationship between Dalixiang and Tropical Japonica. This study reveals the characteristic information of Dalixiang chloroplast genome, and identifies the phylogenetic status of Dalixiang.

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[V1] 2022-06-07 10:45:19 chinaXiv:202206.00102V1 下载全文
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