摘要:头花杜鹃(Rhododendron capitatum)和陇蜀杜鹃(R. przewalskii)是极具观赏价值的 野生花卉和药用植物。为探讨头花杜鹃和陇蜀杜鹃叶绿体基因组的遗传结构及进化特征,该 研究利用 Illumina HiSeq 4000 平台对头花杜鹃和陇蜀杜鹃的叶绿体全基因组进行测序,经 组装和注释后,结合 7 个已发表的杜鹃属植物叶绿体全基因组进行比较基因组学分析和系 统发育分析。结果表明:(1)头花杜鹃和陇蜀杜鹃叶绿体全基因组呈典型的环状四分体结 构,均由一个大单拷贝区(105 990、109 191 bp)、一个小单拷贝区(2 617、2 606 bp)和 一对反向重复区(45 825、47 516 bp)构成,全长分别为 200 257、206 829 bp。(2)头花 杜鹃和陇蜀杜鹃叶绿体基因组中共鉴定出 263 个SSR 位点,大部分 SSR 偏好使用 A/T 碱 基;密码子偏好使用 A/U 结尾。(3)杜鹃属植物叶绿体全基因组中普遍存在基因丢失以 及基因组重排等结构变异现象。该研究丰富了杜鹃属植物的基因组资源,为头花杜鹃、陇蜀 杜鹃的育种、遗传进化及系统发育等相关研究提供了理论基础。
英文摘要:Rhododendron capitatum and R. przewalskii are ornamental flowers and medicinal plants. The chloroplast genomes of R. capitatum and R. przewalskii have been sequenced using Illumina HiSeq 4000 platform to explore the evolution features and genetic structure. After assembly and annotation, the chloroplast genome structure of R. capitatum and R. przewalskii were analyzed and compared with that of other Rhododendron plants. The results were as follows: (1) The chloroplast genomes of R. capitatum and R. przewalskii exhibit a typical quadripartite structure, including a large single copy region (105 990, 109 191 bp), a small single copy region (2 617, 2 606 bp), and a pair of inverted repeat regions (45 825, 47 516 bp). And the two Rhododendron chloroplast genomes were 200 257 and 206 829 bp in length, respectively. (2) In addition, the 263 SSRs were detected in the chloroplast genome of R. capitatum and R. przewalskii, most of which had A/T base preference; the codons preferred ending in A/U. (3) The chloroplast genomes of Rhododendron species exhibit structure variation such as gene loss and genomic rearrangement. Our study enriches the genomic resources of Rhododendron plants and provides the theoretical basis for resource exploitation, genetic evolution, breeding, and phylogeny of R. capitatum and R. przewalskii.
[V1] | 2022-07-05 17:05:49 | chinaXiv:202207.00013V1 | 下载全文 |
1. 宁夏河东沙地不同坡度柠条绵鸡儿(Caragana korshinskii)水分利用策略差异 2022-08-09 |
2. 基于Cubist 模型的天山北坡草地鼠群密度时空分布特征 2022-08-09 |
3. 锰胁迫和性别竞争交互处理下沙棘雌雄幼苗生理响应特征 2022-08-05 |
4. 黑老虎内生真菌及根际土壤真菌的群落结构与生态功能分析 2022-08-05 |
5. 土壤因子与华重楼生物量和药效成分含量相关性分析 2022-08-05 |
6. 马铃薯低氮肥胁迫响应TCP 转录因子的鉴定与分析 2022-08-05 |
7. 四种栎属青冈亚属植物叶绿体基因组特征及系统发育研究 2022-08-05 |
8. 广西被子植物新资料 2022-08-05 |
9. 濒危喀斯特专性植物蚬木在中国的潜在适生区预测 2022-08-05 |
10. 半蒴苣苔属植物染色体制片优化及染色体数目和倍性研究 2022-08-05 |