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甘蔗茎叶的化学成分及抗氧化活性研究

摘要:为了研究甘蔗(Saccharum officinarum) 茎叶的化学成分及抗氧化活性。对甘蔗茎叶,以甲醇提取,提取物采用柱色谱(SiO2、Sephadex LH-20、Rp-18) 进行分离纯化,根据质谱和核磁共振技术鉴定所得化合物的结构;通过􀀁DPPH 法测定化合物的清除自由基能力。结果表明:(1) 从甘蔗茎叶部位共分离鉴定􀀁22 个化合物,分别为对羟基苯甲醛(1),对甲氧基桂皮酸(2),􀀁4-甲氧基苯甲醛(3),香草醛(4),4-羟基肉桂酸甲酯(5),对羟基苯甲酸(6),(2-羟基苯基)(苯基)甲酮(7),对甲基苯甲酸(8),咖啡酸甲酯(9),乌头酸􀀁A (10),乌头酸􀀁E (11),5-O-二甲氧基肉桂酰基奎尼酸(12),槲皮素(13),槲皮素-3-O-α-L-阿拉伯糖苷(14),槲皮素-3-O-β-D-吡喃半乳糖苷(15),硫代二丙酸双十八烷基酯(16),α-conidendrin (17),rel-(2α,3β)-7-O-methylcedrusin(18),3-O-阿魏酰奎宁酸甲酯(19),木犀草素(20),(5S,6S)-5,6-dihydro-3,8,10-trihydroxy-5-(4-hydroxy-3-methoxyphenyl)-6-hydroxymethyl-2, 4-dimethoxy-7H-benzo[c]xanthen-7-one) (21) 和5-O-阿魏酰奎宁酸甲酯(22),其中化合物2-3,7-11,14-19,21-22为首次从该植物中分离得到。(2) 通过DPPH法对含量大的15个化合物(1-9,11-16) 进行自由基清除能力的筛选,其中化合物12 (5-O-二甲氧基肉桂酰基奎尼酸) 显示了较好的抗氧化活性(IC50值为49.58 μg·mL-1)。该研究丰富了甘蔗抗氧化活性物质基础,为其进一步开发利用提供科学依据。

英文摘要:To study the chemical constituents and antioxidant activity from the stems and leaves of Saccharum officinarum. Twenty-two compounds were isolated and purified from the MeOH part of the stems and leaves of S. officinarum by means of various column chromatographic techniques, including silica gel, Sephadex LH-20 and Rp-18 silica gel. Their structures were identified by mass spectrometry and nuclear magnetic resonance; The DPPH method was used to determine the scavenging ability of the components from S. officinarum. The results were as follow: (1) The compounds were identified as p-hydroxybenzaldehyde (1), p-methoxycinnamic acid (2), 4-methoxybenzaldehyde (3), vanillin (4), 4-hydroxy-cinnamic acid methylester (5), p-hydroxyben-zoic acid (6), (2-Hydroxyphenyl)(phenyl)methanone (7), p-methylbenzoic acid (8), caffeic acid methyl ester (9), aconitate A (10), aconitate E (11), 5-O-dimethoxycinnamoylquinic acid (12), quercetin (13),quercetin-3-O-α-L-arabinoside (14), quercetin-3-O-β-D-galactopyranoside (15), didodecyl thiodipropionate (propionic acid, 3,3-sulfinyl di-1,1ʹ-didodecyl ester) (16), α-conidendrin (17), rel-(2α,3β)-7-O-methylcedrusin (18), 3-O-Ferulylquinic acid methyl ester (19), luteolin (20), (5S,6S)-5,6-dihydro-3,8,10-trihydroxy-5-(4-hydroxy-3-methoxyphenyl)-6-hydroxymethyl-2,4- dimethoxy-7H-benzo[c]xanthen-7-one) (21), 3-O-Ferulylquinic acid methyl ester (22). Compounds 2-3, 7-11, 14-19, 21-22 were isolated from this plant for the first time. (2) Determination of free radical scavenging ability of 15 compounds were selected by DPPH method. Compound 12 (5-O-dimethoxycinnamoylquinic acid) had great antioxidant activity (IC50 value was 49.58 μg·mL-1). This study enrich the material basis of antioxidant activity of S. officinarum, which provided a scientific basis for the further development of S. officinarum.

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[V1] 2022-07-05 17:05:48 chinaXiv:202207.00030V1 下载全文
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