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四种栎属青冈亚属植物叶绿体基因组特征及系统发育研究

Comparison of chloroplast genomes and phylogenic analysis of four Species of Quercus subg. Cyclobalanopsis

摘要:青冈亚属植物的系统发育地位长期存在着争议,部分种的种间关系不明确。为揭示宁 冈青冈(Quercus ningangensis)、曼青冈(Q. oxyodon)、毛曼青冈(Q. gambleana)、竹 叶青冈(Q. neglecta)的叶绿体基因组特征及系统发育关系,本研究选择以上4 种青冈的成 熟叶片进行二代测序,对其叶绿体基因组结构和特征进行分析,并结合相关类群进行系统发 育研究。结果表明:(1)宁冈青冈、曼青冈、毛曼青冈、竹叶青冈的叶绿体基因组序列长 度分别为:160 906 bp、160 883 bp、160 832 bp、160 784 bp,它们均编码133 个基因,包括 88 个蛋白质编码基因、37 个tRNA 基因、8 个rRNA 基因。(2)四种青冈更偏好以A/T 结 尾的密码子,质体基因组变异区域主要存在于非编码区域。(3)通过IR 边界分析得出,四 种青冈亚属植物存在ycf1 假基因,且在IRb/SSC 区域发生扩张。(4)系统发育分析表明, 在壳斗科中,水青冈属(Fagus)和三棱栎属(Trigonobalanus)较早分化出来,栎亚属(subg. Quercus)未形成一个单系群,叶绿体基因组建树结果和核标记、质体标记一致,栎亚属中 Ilex 组和Cerris 组间出现穿插现象。(5)《中国植物志》及部分地方植物志将毛曼青冈作 为一个单独的种处理,而周浙昆、邓敏等人根据叶的特征将毛曼青冈作为曼青冈的一个亚种 处理,毛曼青冈的系统发育地位存在着争议,本实验根据叶绿体基因组信息并结合前人的形 态分析结果,支持毛曼青冈作为一个独立的种存在的观点。该研究为探讨青冈亚属系统发育 地位、青冈亚属组的划分、存疑种种间关系的解决提供了基础资料。

英文摘要:The phylogenetic status of Quercus subg. Cyclobalanopsis has long been controversial and the interspecific relationships of some species are unclear. At present, chloroplast genomes have been used to solve the phylogenetic problems of subg. Quercus and Castanea in the Fagaceae, but there are few researches on solving the phylogenetic problems of Quercus subg. Cyclobalanopsis with chloroplast genome information. To reveal the chloroplasts genome characteristics and phylogenetic relationships of Q. ningangensis, Q. oxyodon, Q. gambleana and Quercus neglecta. In this study, mature leaves of the above four species of Quercus subg. Cyclobalanopsis were selected for Next-generation sequencing, and the chloroplast genome structure and characteristics were analyzed, Phylogeny was studied in combination with related taxa. The results were as follows: (1) The chloroplast genome sequences of Q. ningangensis, Q. oxyodon, Q. gambleana and Q. neglecta were 160 906 bp, 160 883 bp, 160 832 bp and 160 784 bp, respectively. They all encoded 133 genes, including 88 protein-coding genes, 37 tRNA genes and 8 rRNA genes. (2) The codons ending in A/T were preferred by the four species of Quercus subg. Cyclobalanopsis, and the variation regions of plastid genome mainly existed in non-coding regions. (3) According to IR boundary analysis, ycf1 pseudogene was found in four species of Quercus subg. Cyclobalanopsis and expanded in IRb/SSC regions. (4) Phylogenetic analysis showed that Fagus and Trigonobalanus were differentiated early in Fagaceae, while subg Quercus did not form a monophyly. The results of phylogenetic tree based on chloroplast genome were consistent with the nuclear marker plasmid markers. Interspersed between Ilex group and Cerris group in subg Quercus. (5) The Flora of China and some local flora treated Q. gambleana as a separate species, while Zhou Zhekun and Deng Min et al. treated Q. gambleana as a subspecies according to the characteristics of leaves. The phylogenetic status of Q. gambleana is still controversial. Based on the chloroplast genome information and previous morphological analysis results, the present study supported the idea that Q. gambleana existed as an independent species. This study provides basic data for discussing the phylogenetic status of the Quercus subg. Cyclobalanopsis, the division of its groups, and the resolution of doubtful relationships among them.

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[V1] 2022-08-05 16:38:28 chinaXiv:202208.00040V1 下载全文
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