Surveillance of SARS-CoV-2 at the Huanan Seafood Market
Emerging in December 2019, coronavirus disease 2019 (COVID-19) eventually became a pandemic and posed a tremendous threat to global public health. However, the origins of SARS-CoV-2, the causative agent of COVID-19, remain to be determined. It has been reported that a certain number of the early case clusters had a contact history with the Huanan Seafood Market. Therefore, surveillance of SARS-CoV-2 within the market is of vital importance. Herein, we presented the SARS-CoV-2 detection results of 1380 samples collected from the environment since 1st Jan and animals since 18th Jan within the market in early 2020. By SARS-CoV-2-specific RT-qPCR, 73 environmental samples tested positive for SARS-CoV-2 and three live viruses were successfully isolated. The viruses from the market shared nucleotide identity of 99.99% to 100% with the human isolate HCoV-19/Wuhan/IVDC-HB-01/2019. The A lineage (8782T and 28144C), as the likely ancestral SARS-CoV-2 lineage, was found in an environmental sample. No virus was detected in the animal swabs covering 18 species of animals in the market. The RNA-seq analysis of SARS-CoV-2 positive/negative environmental samples showed the abundance of different vertebrata genera. In summary, this study provided convincing evidence of the prevalence of SARS-CoV-2 in the Huanan Seafood Market during the early stage of COVID-19 outbreak.
William J. Liu,Peipei Liu,Wenwen Lei, Zhiyuan Jia,Xiaozhou He,Weifeng Shi,Yun Tan, Shumei Zou, Gary Wong,Ji Wang,Feng Wang,Gang Wang,Kun Qin,Rongbao Gao, Jie Zhang, Min Li,Wenling Xiao,Yuanyuan Guo,Ziqian Xu,Yingze Zhao,Jingdong Song,Jing Zhang,Wei Zhen,Wenting Zhou,Beiwei Ye,Juan Song,Mengjie Yang,Weimin Zhou,Yuting Dai,Gang Lu,Yuhai Bi,Wenjie Tan,Jun Han,George F. Gao,Guizhen Wu.(2023).Surveillance of SARS-CoV-2 at the Huanan Seafood Market.CSTR:32003.36.ChinaXiv.202303.10351.V1