A micro E-DNA sensor for selective detection of dopamine in presence of ascorbic acid

• 作者： ZHU Dan ^1,2 LI Min ^1,2 WANG Li-Hua ¹ ZUO Xiao-Lei ¹
• 作者单位：
  1.  Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
  2.  University of Chinese Academy of Sciences, Beijing 100049, China
• 提交时间：2023-06-18

摘要: In this paper, a novel method for selectively detection of dopamine (DA) in the interference of ascorbic acid (AA) is described. A nanometer-sized gold flower microelectrode (NGFME) is prepared by flame-etching and electrochemical deposition. The electrode tip was characterized by scanning electron microscope (SEM). The NGFME is sized at about 100 m and dimensions of thorns of the electrode were in nanometers. By modifying with DA aptamer on the surface, the prepared aptasensor can selectively detect DA even in the presence of high concentration AA. Experimental results show that this NGFME has no response to AA. As a comparison, the carbon fiber electrode without DA aptamer modification is unable to effectively detect DA in the presence of AA. The NGFME is easy-to-prepare, selective and sensitive for DA detection down to 25 m. The electrode can be expected to detect DA in vivo and in real biological samples.

• 期刊： Nuclear Science and Techniques
• 分类： 物理学 >> 核物理学
• 投稿状态： 已在期刊出版
• 引用： ChinaXiv:202306.00207 (或此版本 ChinaXiv:202306.00207V1)
  DOI:dx.doi.org/10.13538/j.1001-8042/nst.26.060504
  CSTR:32003.36.ChinaXiv.202306.00207.V1
• 推荐引用方式： ZHU Dan,LI Min,WANG Li-Hua,ZUO Xiao-Lei.(2023).A micro E-DNA sensor for selective detection of dopamine in presence of ascorbic acid.Nuclear Science and Techniques.doi:dx.doi.org/10.13538/j.1001-8042/nst.26.060504 (点此复制)