分类: 生物学 >> 生物物理学 提交时间: 2016-05-11
摘要: MicroRNA-155 (miR-155) plays significant role in various physiological processes involving both innate and adaptive immunity. miR-155 expression level changes dynamically during various immune responses. However, current approaches for miR-155 detection at the RNA level do not precisely reflect the real-time activity. Herein, we generated a transgenic mouse line (R26-DTR-155T) for determination of miR-155-5p activity in vivo by inserting miR-155-5p target sequence downstream of a reporter transgene comprising Diphtheria Toxin Receptor and TagBlue fluorescence protein. Using this approach, R26-DTR-155T mice were able to measure variation in levels of miR-155-5p activity in specific cell types of interest. The DTR expression levels were inversely correlated with the endogenous miR-155 expression pattern as detected by quantitative RT-PCR. Our data demonstrate a novel transgenic mouse line which could be useful for tracing miR-155-5p activity in specific cell types through measurement of miR-155-5p activity at single cell level.
分类: 天文学 >> 天文学 提交时间: 2024-03-29 合作期刊: 《Research in Astronomy and Astrophysics》
摘要: Cloud cover plays a pivotal role in assessing observational conditions for astronomical site-testing. Except for the fraction of observing time, its fragmentation also wields a significant influence on the quality of nighttime sky clarity. In this article, we introduce the function , designed to comprehensively capture both the fraction of available observing time and its continuity. Leveraging in situ measurement data gathered at the Muztagh-Ata site between 2017 and 2021, we showcase the effectiveness of our approach. The statistical result illustrates that the Muztagh-Ata site affords approximately 122 nights that were absolutely clear and 205 very good nights annually, corresponding to Γ ≥ 0.9 and Γ ≥ 0.36 respectively.