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1. chinaXiv:202002.00071 [pdf]

Potential treatment of Chinese and Western Medicine targeting nsp14 of 2019 nCov

Liu Chao ; Zhu Xiaoxiao ; Lu Yiyao ; Jia Xu ; Yang Tai
Subjects: Medicine, Pharmacy >> Pharmacology

2019年12月,自武汉开始我国爆发严重的新型冠状病毒(2019-nCoV)感染疫情,急需开发2019-nCoV治疗药物。冠状病毒非结构蛋白 (Nonstructural protein, NSP) 在病毒基因组复制以及转录过程中起重要作用,在冠状病毒家族中普遍保守,是冠状病毒重要功能蛋白。其中,NSP14蛋白兼具核酸外切酶和甲基转移酶功能,是抗SARS和其他冠状病毒药物研究的重要靶点。序列分析表明, SARS coronavirus (PDB ID: 5nfy) 与武汉华南海鲜批发市场新型冠状病毒分离毒株 (GenBank: MN985325.1)氨基酸序列同源性为98.7%。本研究通过已发表的SARS-NSP14晶体结构 (PDB ID: 5nfy) 进行同源建模,构建2019-nCoV-NSP14的蛋白三维结构模型,对其N端-甲基转移酶以及C端-甲基转移酶结构功能域分别进行虚拟筛选,筛选出了18个对NSP14有抑制活性的化合物。其中沙奎那韦 (Saquinavir)、溴隐亭 (Bromocriptine)、黄芩苷 (Baicalein) 以及金丝桃素 (Hypericin) 能同时作用于NSP14蛋白上述两个重要功能域。该结果提示已知抗HIV药物-Saquinavir以及中药抗病毒重要活性成分-黄芩苷以及金丝桃素,可能以2019-nCoV-NSP14蛋白为靶标,发挥抗病毒效应,可作为抗2019-nCov候选药物进行下一步研究。

submitted time 2020-02-27 Hits33357Downloads2913 Comment 0

2. chinaXiv:201605.01503 [pdf]

Effects of application of corn straw on soil microbial community structure during the maize growing season

Lu, Ping; Lin, Yin-Hua; Xu, Yan-Peng; Tan, Fei; Jia, Xu-Dong; Lu, Ping; Yang, Zhong-Qi; Wang, Xi-Zhuo; Xu, Yan-Peng; Tan, Fei; Wang, Miao; Xu, De-Rong
Subjects: Biology >> Biophysics

This study investigated the influence of corn straw application on soil microbial communities and the relationship between such communities and soil properties in black soil. The crop used in this study was maize (Zea mays L.). The five treatments consisted of applying a gradient (50, 100, 150, and 200%) of shattered corn straw residue to the soil. Soil samples were taken from May through September during the 2012 maize growing season. The microbial community structure was determined using phospholipid fatty acid (PLFA) analysis. Our results revealed that the application of corn straw influenced the soil properties and increased the soil organic carbon and total nitrogen. Applying corn straw to fields also influenced the variation in soil microbial biomass and community composition, which is consistent with the variations found in soil total nitrogen (TN) and soil respiration (SR). However, the soil carbon-to-nitrogen ratio had no effect on soil microbial communities. The abundance of PLFAs, TN, and SR was higher in C1.5 than those in other treatments, suggesting that the soil properties and soil microbial community composition were affected positively by the application of corn straw to black soil. A Principal Component Analysis indicated that soil microbial communities were different in the straw decomposition processes. Moreover, the soil microbial communities from C1.5 were significantly different from those of CK (p<0.05). We also found a high ratio of fungal-to-bacterial PLFAs in black soil and significant variations in the ratio of monounsaturated-to-branched fatty acids with different straw treatments that correlated with SR (p<0.05). These results indicated that the application of corn straw positively influences soil properties and soil microbial communities and that these properties affect these communities. The individual PLFA signatures were sensitive indicators that reflected the changes in the soil environment condition.

submitted time 2016-05-12 Hits2074Downloads1000 Comment 0

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