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1. chinaXiv:201605.01282 [pdf]

Histone H1-mediated epigenetic regulation controls germline stem cell self-renewal by modulating H4K16 acetylation

Sun, Jin; Xu, Jiang; Yang, Zhihao; Ren, Xingjie; Lv, Wen-Wen; Liu, Lu-Ping; Pan, Li-Xia; Wang, Xia; Qiao, Huan-Huan; Ni, Jian-Quan; Wei, Hui-Min; Chang, Jian-Feng; Sun, Fang-Lin; Xu, Jiang; Liu, Lu-Ping; Zhu, Bing; Ji, Jun-Yuan; Yan, Dong; Xie, Ting; Xie, Ting
Subjects: Biology >> Biophysics

Epigenetics plays critical roles in controlling stem cell self-renewal and differentiation. Histone H1 is one of the most critical chromatin regulators, but its role in adult stem cell regulation remains unclear. Here we report that H1 is intrinsically required in the regulation of germline stem cells (GSCs) in the Drosophila ovary. The loss of H1 from GSCs causes their premature differentiation through activation of the key GSC differentiation factor bam. Interestingly, the acetylated H4 lysine 16 (H4K16ac) is selectively augmented in the H1-depleted GSCs. Furthermore, overexpression of mof reduces H1 association on chromatin. In contrast, the knocking down of mof significantly rescues the GSC loss phenotype. Taken together, these results suggest that H1 functions intrinsically to promote GSC self-renewal by antagonizing MOF function. Since H1 and H4K16 acetylation are highly conserved from fly to human, the findings from this study might be applicable to stem cells in other systems.

submitted time 2016-05-11 Hits1599Downloads959 Comment 0

2. chinaXiv:201605.00404 [pdf]

The Arabidopsis CROWDED NUCLEI genes regulate seed germination by modulating degradation of ABI5 protein

Wenming Zhao; Chunmei Guan; Jian Feng; Yan Liang; Ni Zhan; Jianru Zuo; Bo Ren
Subjects: Biology >> Botany >> Plant physiology

In Arabidopsis, the phytohormone abscisic acid (ABA) plays a vital role in inhibiting seed germination and in post-germination seedling establishment. In the ABA signaling pathway, ABI5, a basic Leu zipper transcription factor, has important functions in the regulation of seed germination. ABI5 protein localizes in nuclear bodies, along with AFP, COP1, and SIZ1, and was degraded through the 26S proteasome pathway. However, the mechanisms of ABI5 nuclear body formation and ABI5 protein degradation remain obscure. In this study, we found that the Arabidopsis CROWDED NUCLEI (CRWN) proteins, predicted nuclear matrix proteins essential for maintenance of nuclear morphology, also participate in ABA-controlled seed germination by regulating the degradation of ABI5 protein. During seed germination, the crwn mutants are hypersensitive to ABA and have higher levels of ABI5 protein compared to wild type. Genetic analysis suggested that CRWNs act upstream of ABI5. The observation that CRWN3 colocalizes with ABI5 in nuclear bodies indicates that CRWNs might participate in ABI5 protein degradation in nuclear bodies. Moreover, we revealed that the extreme C-terminal of CRWN3 protein is necessary for its function in the response to ABA in germination. Our results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination.

submitted time 2016-05-03 Hits4368Downloads1146 Comment 0

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