分类： 医学、药学 >> 预防医学与公共卫生学 提交时间： 2023-03-29
William J. Liu
Peipei Liu
Wenwen Lei
Zhiyuan Jia
Xiaozhou He
Weifeng Shi
Yun Tan
Shumei Zou
Gary Wong
Ji Wang
Feng Wang
Gang Wang
Kun Qin
Rongbao Gao
Jie Zhang
Min Li
Wenling Xiao
Yuanyuan Guo
Ziqian Xu
Yingze Zhao
摘要：Emerging in December 2019, coronavirus disease 2019 (COVID-19) eventually became a pandemic and posed a tremendous threat to global public health. However, the origins of SARS-CoV-2, the causative agent of COVID-19, remain to be determined. It has been reported that a certain number of the early case clusters had a contact history with the Huanan Seafood Market. Therefore, surveillance of SARS-CoV-2 within the market is of vital importance. Herein, we presented the SARS-CoV-2 detection results of 1380 samples collected from the environment since 1st Jan and animals since 18th Jan within the market in early 2020. By SARS-CoV-2-specific RT-qPCR, 73 environmental samples tested positive for SARS-CoV-2 and three live viruses were successfully isolated. The viruses from the market shared nucleotide identity of 99.99% to 100% with the human isolate HCoV-19/Wuhan/IVDC-HB-01/2019. The A lineage (8782T and 28144C), as the likely ancestral SARS-CoV-2 lineage, was found in an environmental sample. No virus was detected in the animal swabs covering 18 species of animals in the market. The RNA-seq analysis of SARS-CoV-2 positive/negative environmental samples showed the abundance of different vertebrata genera. In summary, this study provided convincing evidence of the prevalence of SARS-CoV-2 in the Huanan Seafood Market during the early stage of COVID-19 outbreak.
分类： 生物学 >> 病毒学 分类： 医学、药学 >> 基础医学 提交时间： 2021-07-28
Qihui Wang
Hua Chen
Yi Shi
Alice C. Hughes
William J. Liu
Jingkun Jiang
George F. Gao
Yongbiao Xue
Yigang Tong
摘要： 新冠病毒的起源仍不清楚。了解新冠病毒如何、何时以及在何处从其天然宿主传播给人类对于预防未来由冠状病毒引发的疫情至关重要。 从与病原体无休止的战斗中吸取教训，结合目前已知的关于新冠病毒起源和中间宿主的研究数据，我们提出全球多个地点都有可能是新冠病毒的起源地。
分类： 生物学 >> 生物化学 提交时间： 2020-02-12
Yingxia Liu
Cong Zhang
Fengming Huang
Yang Yang
Fuxiang Wang
Jing Yuan
Zheng Zhang
Yuhao Qin
Xiaoyun Li
Dandan Zhao
Shunwang Li
Shuguang Tan
Zhaoqin Wang
Jinxiu Li
Chenguang Shen
Jianming Li
Ling Peng
Weibo Wu
Mengli Cao
Li Xing
摘要： 最近，在中国武汉爆发了肺炎病例，由一种名为2019-CoV的新型冠状病毒引起。我们之前的研究中，根据中国深圳的12例2019-nCoV感染患者的临床特征，所有病例均患有肺炎，一半病例发展为急性呼吸窘迫综合征（ARDS）。本文中，我们针对这12例患者的血浆因子表达谱进行了研究。我们测试了2019-nCoV感染患者血浆中的48个因子的表达，其中38个因子与健康个体相比显着升高；2019-nCoV感染的重症患者血浆中的高细胞因子血症水平显著低于A型流感病毒H7N9感染患者，而略高于细菌感染患者。在这38个细胞因子中，有17个与2019-CoV病毒载量相关，其中的15个（M-CSF，IL-10，IFN-α2，IL-17，IL-4，IP-10，IL-7，IL-1受体拮抗剂，G-CSF，IL-12 （p40），IFN-γ，IL-1α，IL-2，HGF和PDGF-BB）与肺损伤Murray评分高度相关，可用来预测2019-nCoV感染患者的疾病严重程度。我们的研究结果表明，这15种高细胞因子可能是衡量2019-nCoV感染患者疾病严重程度的潜在生物标志物，影响这些信号传递介质的因子可能是针对新型2019-nCoV大流行的潜在药物。
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