分类： 医学、药学 >> 基础医学 提交时间： 2023-05-22
Qin, Lijuan
Wang, Jiuru
Cheng, Fang
Zhang, Han
Zheng, Huaguo
Liu, Yongai
Liang, Zhentao
Wang, Baifeng
Wang, Haoyu
Ju, Ying
Tian, Huaqin
Meng, Songdong
摘要：Songdong MENG conceived of the project. Huaqin TIAN, Ying JU supervised the project. Lijuan QIN, Fang CHENG, and Jiuru WANG performed the experiments and analyzed the data. Lijuan QIN edited the original draft. Yongai LIU, Han ZHANG, Huaguo ZHENG, Baifeng WANG, Zhentao LIANG, and Haoyu WANG assisted with performing the experiments. Songdong MENG, Lijuan QIN, and Ying JU wrote the manuscript.
分类： 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间： 2016-05-11
Cui, Feng
Chang, Hai-Jing
Mao, Yun
Zhang, Han-Ying
Lu, Li-Xia
Yan, Shuai-Guo
Qiao, Chuan-Ling
Li, Mei-Xia
Liu, Li
Chang, Hai-Jing
Lang, Ming-Lin
Mao, Yun
Yan, Shuai-Guo
摘要：Carboxylesterases are mainly involved in the mediation of metabolic resistance of many insects to organophosphate (OP) insecticides. Carboxylesterases underwent two divergent evolutionary events: (1) quantitative mechanism characterized by the overproduction of carboxylesterase protein; and (2) qualitative mechanism caused by changes in enzymatic properties because of mutation from glycine/alanine to aspartate at the 151 site (G/A151D) or from tryptophan to leucine at the 271 site (W271L), following the numbering of Drosophila melanogaster AChE. Qualitative mechanism has been observed in few species. However, whether this carboxylesterase mutation mechanism is prevalent in insects remains unclear. In this study, wild-type, G/A151D and W271L mutant carboxylesterases from Culex pipiens and Aphis gossypii were subjected to germline transformation and then transferred to D. melanogaster. These germlines were ubiquitously expressed as induced by tub-Gal4. In carboxylesterase activity assay, the introduced mutant carboxylesterase did not enhance the overall carboxylesterase activity of flies. This result indicated that G/A151D or W271L mutation disrupted the original activities of the enzyme. Less than 1.5-fold OP resistance was only observed in flies expressing A. gossypii mutant carboxylesterases compared with those expressing A. gossypii wild-type carboxylesterase. However, transgenic flies universally showed low resistance to OP insecticides compared with non-transgenic flies. The flies expressing A. gossypii W271L mutant esterase exhibited 1.5-fold resistance to deltamethrin, a pyrethroid insecticide compared with non-transgenic flies. The present transgenic Drosophila system potentially showed that a quantitative increase in carboxylesterases induced broader resistance of insects to insecticides than a qualitative change. (C) 2014 Elsevier Inc. All rights reserved.
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