Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Plant Protection submitted time 2018-06-22 Cooperative journals: 《广西植物》
Abstract： Phytagel and sucrose have great influence on the regeneration efficiency of somatic embryos and growth of regenerated plants. In this study, effects of Phytagel and sucrose on the regeneration of somatic embryos and growth of regenerated plants were carried out on mature cotyledonary embryos from rubber tree clone Reyan7-33-97. MS medium supplemented with 0.23 μmol• L-1 KT, 0.11 μmol• L-1 IAA and 8.7 μmol• L-1 GA3 was used as the plant regeneration medium. The results showed that appropriate Phytagel was helpful for the regeneration efficiency of somatic embryos and growth of regenerated plants. At lower concentrations (0-1 g•L-1), the frequency of plant regeneration increased with the increase in the amount of Phytagel, but at higher concentrations (1-4 g•L-1), along with the Phytagel with increasing amounts, plant growth was inhibited. When the Phytagel was supplemented in somatic embryo regeneration medium with 1 g•L-1, the plant regeneration rate, the plants (height >5 cm) and the plants with leaves reached 86.4 ± 5.7%, 53 ± 9.4% and 81.7 ± 3%, respectively, higher than other treatments;
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