Subjects: Biology >> Botany >> Applied botany submitted time 2021-05-11 Cooperative journals: 《广西植物》
Abstract： The dried roots of Sophora tonkinensis were commonly used as the treatment of the throat and other infectious diseases. In order to identify its large polar antibacterial active components, silica gel column chromatography (CC), ODS CC and Sephadex LH-20 CC and semi preparative HPLC were used to isolate the chemical constituents from n-butanol fraction of S. tonkinensis. The antibacterial activities of the monomers against bacteria (Enterobacter faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli) and fungi (Candida albicans) were evaluated. The results showed ten compounds were isolated and identified as 2',4',7-trihydroxy-6,8-bis(3-methyl-2-butenyl)flavanone(1),genistin(2), 2-2',4'-dihydroxy-5,6-dioxomethylbenzofuran(3), maltol-β-D-glucopyranoside(4), leonuriside A(5),coniferylalcohol-9-O-β-D-glucopyranoside(6),glabrol(7),2-(2',4'-dihydroxy-phenyl)-8,8-dimethyl-10-(3-methyl-2-butenyl)-8H-pyrano[2,3-d]chroman-4-one (8), oxymatrine (9), piscidic acid (10). Among of them, compound 4-6 was isolated from the title plant for the first time, for our knowledge. Besides, biological activity test showed the potential antibacterial activities of compounds 1-3. It is worth mentioning that the minimum inhibitory concentration (MIC) value of compound 2 against Pseudomonas aeruginosa was 15.6 μg·mL-1, while the MIC of compound 3 against Candida albicans and Escherichia coli were 31.3 μg·mL-1.
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