Subjects: Biology >> Botany >> Applied botany submitted time 2022-08-05 Cooperative journals: 《广西植物》
Abstract： Phylogenetic relationships of Scrophulariaceae s. l.（sensu lato）and the closely related groups were explored with 129 plastid genomes representing 107 genera. Plastome sequences were downloaded from the GenBank. Based on coding sequences (CDS), phylogenetic trees were reconstructed using maximum likelihood （ML）and Bayesian inference（BI）approaches. The ML and BI analyses shared the same topology and the main clades were consistently recovered with high support. Of the 129 total nodes, 123 were supported by ML bootstrap value ≥70%. The resulting phylogenies were as follows：（1）Scrophulariaceae s. l. was confirmed as polyphyletic. Fifty-one species with representatives of 37 genera from the Scrophulariaceae s.l. were assigned to seven families. These were Orobanchaceae, Paulowniaceae, Wightiaceae, Mazaceae, Linderniaceae, Scrophulariaceae s.s.（sensu stricto）, and Plantaginaceae. （2）Scrophulariaceae s.s. was a monophyletic group. In addition to the recognition of eight genera （ including Bontia 、Calamphoreus 、Diocirea 、 Eremophila、Glycocystis、Leucophyllum、Scrophularia and Verbascum）comprised primarily of former members of Scrophulariaceae s.l., Buddleja which was originally placed in Loganiaceae, and Myoporum, traditionally placed in the Myoporaceae had been demonstrated to belong to this clade. （3）The Lamiales was monophyletic. Fourteen well-supported monophyletic clades were obtained corresponding to each family (Wightiaceae and Pedaliaceae for which only one species had been contained were not included). The relationships among the families were well resolved. Oleaceae formed a well-supported clade that was distinct from core Lamiales. Within the core Lamiales, four clades (Gesneriaceae, Plantaginaceae, Scrophulariaceae, and Linderniaceae) diverged before the remaining families grouping in two sister clades. Within one clade, two moderately to highly supported subclades were recovered. Lentibulariaceae was resolved as sister group to Acanthaceae, and Pedaliaceae was sister to Verbenaceae + Bignoniaceae. The other clade contained six families, with Lamiaceae, Mazaceae, Wightiaceae + Phrymaceae, and Paulowniaceae successively sister to Orobanchaceae. The results of our analyses indicate that the traditional Scrophulariaceae are an unnatural assemblage of plants distributed throughout the phylogenentic tree of Lamiales. Phylogenomics analyses based on plastid organelle have shown good potential for phylogenetic inference in Scrophulariaceae, more taxonomic sampling are needed to enhance our understanding of phylogenetic relationships of Scrophulariaceae in future studies.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-10-17 Cooperative journals: 《广西植物》
Abstract：该研究基于叶绿体基因组数据，对桃金娘目（6科44属97种）及其近缘类群（牻牛儿苗目2科5属25种）的系统发育关系进行了分析。结果表明：（1）桃金娘目基因组大小为15.2~17.1 kb，包括的蛋白质编码基因数目为74~90个，牻牛儿苗目基因组大小为11.6~24.2 kb，包括的蛋白质编码基因数目为75~132个；（2）对比叶绿体基因组序列和蛋白质编码基因所构建的系统发育树结果，在目间及牻牛儿苗目内差异显著，桃金娘目内基本一致；（3）基于蛋白质编码基因所构建的系统发育树表明，桃金娘目和牻牛儿苗目均为单系，为姐妹类群；桃金娘目内形成两个大支，桃金娘科、Vochysiaceae、野牡丹科形成一支，其中桃金娘科和Vochysiaceae关系较近是姐妹群，柳叶菜科、千屈菜科和使君子科形成另一支，其中柳叶菜科和千屈菜科关系较近为姐妹群；科级水平，桃金娘科、Vochysiaceae、野牡丹科、柳叶菜科、千屈菜科、使君子科和牻牛儿苗科均为单系（仅包括一个物种的科除外）；（4）支持将石榴属及菱属置于千屈菜科；（5）对蛋白质编码基因序列变异分析的结果表明，野牡丹科19个属的共享变异基因数目为53个，变异百分数范围为5.84%~29.53%，桃金娘科9个属的共享变异基因数目为57个，其变异百分数范围为1.31%~15.78%。本研究结果为进一步研究桃金娘目及相关科属的系统发育提供了理论依据。
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