Subjects: Biology >> Botany submitted time 2023-01-30 Cooperative journals: 《广西植物》
Abstract：
In order to find culturable bacteria resources and antibacterial activity strain in the rhizosphere soil of mangrove plants from the Maowei Sea, the rhizosphere soil of five mangrove plants(Rhizophora stylosa, Talipariti tiliaceum, Sonneratia apetala, Aegiceras corniculatum, and Pluchea indica) was cultured by enrichment media and then isolated the culturable bacteria. The culturable bacteria were purified by six different isolation media. The diversity of culturable bacteria was analyzed by the 16S rRNA gene sequence information. Antimicrobial activities (Staphylococcus epidermidis, Methicillin-resistant Staphylococcus aureus, and Pseudomonas aeruginosa) of the culturable bacteria were analyzed by disk diffusion method. The results were as follows: (1) A total of 120 strains were isolated from 7 rhizosphere soil samples of mangrove plants which could be classified into 35 families and 47 genera, among which 5 potential new species were obtained. The dominat genus were Streptomyces (14.2%). (2) It was found that the crude extracts of 9 strains had antibacterial activity against at least one pathogen. The results indicate that there are abundant culturable bacteria and potential new antibiotics resources in the mangrove rhizosphere soils from the Maowei Sea.
Subjects: Biology >> Botany >> Applied botany submitted time 2021-06-02 Cooperative journals: 《广西植物》
Abstract： This study aims to explore potential new bacterial species and bioactive substances in mangrove plants, which can enrich the diversity of mangrove microorganisms and provide strain resources for the development of new active products. 22 samples were collected from three true mangrove plants of Kandelia candel, Bruguiera gymnorrhiza, Rhizophora stylosa, and their habitats, in Guangxi coastal area. Then 22 samples were divided according to different parts such as roots, stems, leaves, flowers, fruits and muds. Eight different culture mediums were used to isolate culturable bacteria, and 16S rRNA gene sequences were used to identify bacteria and analyze diversity. For cultured bacteria, the antibacterial and enzyme activities of fermented crude extracts were screened with Kirby-Bauer method and spot planting method, respectively. The results were as follows:（1）Based on sequencing results of 16S rRNA gene, a total of 35 culturable bacteria were isolated, belonging to 28 genera and 23 families. Bacillus accounted for 14.3% of the total bacteria, which was the dominant bacteria. Meanwhile, 11 potential new bacterial species were found. （2）Four strains of bacteria showed antibacterial activities, 16 strains had enzyme activities, and Bacillus was the dominant strain in enzyme activities. The above results showed that Guangxi true mangrove plants were rich in bacterial diversity, and some bacteria showed antibacterial activities and enzyme activities. The new bacterial species and active strain had certain development potential in the new antibiotics and enzymes application.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-08-26 Cooperative journals: 《广西植物》
Abstract： To study the distribution, diversity and nematicidal activity of endophytic bacteria from mangrove plants collected from Beibu Gulf coast at Xuwen. It can provide bacterial resources for the microbial insecticides. The bacteria were isolated from sixteen samples seven mangrove plants which collected from the Xuwen by designed 10 kinds of isolation medias. The 16S rRNA molecular biology method, dilution coating method and Caenorhabditis elegans screening model were used to explore the diversity and nematicidal activity of endophytic bacteria isolated from mangrove plants. The results showed that 33 endophytic bacteria were isolated by 16 organs and tissues which could be classified into 19 families and 23 genera and the dominant genus were Bacillus. The eight potential new species or genera were obtained. Through the nematicidal activity test obtained 2 kinds of activity bacteria. Two endophytic bacterial had the extremely significant nematicidal activities and the lethal concentration of 50% was 28.63 mg·mL-1 and 178.03 mg·mL-1, respectively. The research confirm that mangrove plants collected from the Xuwen are rich in endophytic bacteria. At the same time, parts of the bacteria have strong nematicidal activity, which could be a potential source for the discovery of the new microbial insecticides.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-03-14 Cooperative journals: 《广西植物》
Abstract：设计9种分离培养基，采用稀释涂布法从14份真红树植物的46份组织样品中分离纯化内生细菌。基于菌株形态学特征和16S rRNA基因序列确定分离菌株的种属，并分析其物种多样性，采用秀丽隐杆线虫模型筛选菌株延缓衰老活性。结果表明：通过基因序列去重复后从46份真红树植物组织样品中获得32株海洋细菌，基于菌株16S rRNA基因序列信息分析，覆盖12科17属，其中芽孢杆菌属（Bacillus）为优势菌属，并获得1株疑似橙单胞菌属（Aurantimonas）新种，16S rRNA基因序列相似性低于97%。经过秀丽隐杆线虫粗筛发现3株海洋细菌具有显著延缓线虫衰老的活性（P<0.05）。研究结果表明海南西海岸真红树内生细菌具有物种多样性，部分菌株具有延缓线虫衰老活性。
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