Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Plant Protection submitted time 2018-07-18 Cooperative journals: 《广西植物》
Abstract： Cymbidium tracyanum is a Orchidaceae species, characterized by a beautiful and showy inflorescence. The species has a great potential for commercial exploitation in the flower industry. Plant tissue culture technology offers an effective tool for C. tracyanum large-scale production to avoid endangering its natural populations, yet there is a few research on the plant tissue culture of C. tracyanum. The current study evaluated the effects of basic medium and hormone combination on the plant regeneration of C. tracyanum. The effects of illumination time and culture temperature on the growth of plantlets were also studied. The results showed that the optimum basic medium for C. tracyanum growth was 1/2 MS. The optimal medium for protocorm induction was 1/2 MS＋1.0 mg·L-1 6-BA＋0.5 mg·L-1 NAA, 95.00% of the seeds developed into the protocorms after cultured for 50 d. The optimal medium for protocorm proliferation was 1/2 MS＋2.0 mg·L-1 NAA, the maximum proliferation multiple of protocorm reached 4.25 after cultured for 30 d. The optimal medium for protocorm differentiation was 1/2 MS＋2.0 mg· L-1 NAA＋60 g· L-1 mashed potato＋0.5 g· L-1acticarbon, the induction rate of indefinite bud was 98.33% after cultured for 10 d, the rooting rate was 94.67% after cultured for 40 d. In the process of plantlets subculture, temperature of 20℃ , illumination time of 12 h·d-1 and illumination intensity of 2 000 lx were suited for the growth of plantlets, the physiological scorch rate of leaf tip was 3.33%. Using humus as cultivation substrate, the transplanting survival rate of plantlets was 97.78%. This study provides an effective way for keeping good varieties of features and rapid propagation of C. tracyanum, at the same time helps to solve some theoretical problems in factory production of C. tracyanum.
Hits
Hits
Downloads
Comment