Nanozyme-strip for rapid local diagnosis of Ebola

分类： 生物学 >> 生物物理学 提交时间： 2016-05-11

Duan, Demin Fan, Kelong Zhang, Dexi Zhang, Jianlin Yan, Xiyun Tan, Shuguang Gao, George Fu Liang, Mifang Liu, Yang Zhang, Panhe Liu, Wei Qiu, Xiangguo Kobinger, Gary P. Gao, George Fu

摘要： Ebola continues to rage in West Africa. In the absence of an approved vaccine or treatment, the priority in controlling this epidemic is to promptly identify and isolate infected individuals. To this end, a rapid, highly sensitive, and easy-to-use test for Ebola diagnosis is urgently needed. Here, by using Fe3O4 magnetic nanoparticle (MNP) as a nanozyme probe, we developed a MNP-based immunochromatographic strip (Nanozyme-strip), which detects the glycoprotein of Ebola virus (EBOV) as low as 1 ng/mL, which is 100-fold more sensitive than the standard strip method. The sensitivity of the Nanozyme-strip for EBOV detection and diagnostic accuracy for New Bunyavirus clinical samples is comparable with ELISA, but is much faster (within 30 min) and simpler (without need of specialist facilities). The results demonstrate that the Nanozyme-strip test can rapidly and sensitively detect EBOV, providing a valuable simple screening tool for diagnosis of infection in Ebola-stricken areas. (C) 2015 Elsevier B.V. All rights reserved.

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