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1. chinaXiv:201605.01342 [pdf]

Functional Characterization of Long Noncoding RNA Lnc_bc060912 in Human Lung Carcinoma Cells

Luo, Huaxia; Sun, Yu; Wei, Guifeng; Luo, Jianjun; Yang, Xinling; Liu, Wei; Chen, Runsheng; Luo, Huaxia; Sun, Yu; Wei, Guifeng; Yang, Xinling; Liu, Wei; Chen, Runsheng; Yang, Xinling; Guo, Mingzhou
Subjects: Biology >> Biophysics >> Biochemistry & Molecular Biology

Long noncoding RNAs (lncRNAs) are pervasively transcribed in the human genome. Recent studies suggest that the involvement of IncRNAs in human diseases could be far more prevalent than previously appreciated. Here we have identified a lncRNA termed Lnc_bc060912 whose expression is increased in human lung and other tumors. Lnc_bc060912 is 1.2 kb in length and is composed of two exons. The expression of Lncbc060912, was repressed by p53. Lnc_bc060912 suppressed cell apoptosis. Using a recently developed method for RNA-pulldown with formaldehyde cross-linking, we found that Lnc_bc060912 interacted With the two DNA damage repair proteins PARP1 and NPM1. Together, these results suggest that Lnc_bc060912, via PARP1 and NPM1, affects cell apoptosis and may play important roles in tumorigenesis and cancer progression.

submitted time 2016-05-11 Hits1915Downloads1172 Comment 0

2. chinaXiv:201605.01324 [pdf]

Molecular characteristics and evolutionary analysis of a very virulent infectious bursal disease virus

Li Zan; Zhu Ping; Qi XiaoLe; Ren XianGang; Wang XiaoMei; Cui Lei; Li Zan
Subjects: Biology >> Biophysics >> Biology

Infectious bursal disease virus (IBDV) poses a significant threat to the poultry industry. Viral protein 2 (VP2), the major structural protein of IBDV, has been subjected to frequent mutations that have imparted tremendous genetic diversity to the virus. To determine how amino acid mutations may affect the virulence of IBDV, we built a structural model of VP2 of a very virulent strain of IBDV identified in China, vvIBDV Gx, and performed a molecular dynamics simulation of the interaction between virulence sites. The study showed that the amino acid substitutions that distinguish vvIBDV from attenuated IBDV (H253Q and T284A) favor a hydrophobic and flexible conformation of beta-barrel loops in VP2, which could promote interactions between the virus and potential IBDV-specific receptors. Population sequence analysis revealed that the IBDV strains prevalent in East Asia show a significant signal of positive selection at virulence sites 253 and 284. In addition, a signal of co-evolution between sites 253 and 284 was identified. These results suggest that changes in the virulence of IBDV may result from both the interaction and the co-evolution of multiple amino acid substitutions at virulence sites.

submitted time 2016-05-11 Hits2515Downloads1376 Comment 0

3. chinaXiv:201605.01301 [pdf]

The Long Noncoding RNA IncTCF7 Promotes Self-Renewal of Human Liver Cancer Stem Cells through Activation of Wnt Signaling

Wang, Yanying; Du, Ying; Zhu, Pingping; Huang, Guanling; Yan, Xinlong; Ye, Buqing; Li, Chong; Xia, Pengyan; Zhang, Geng; Fan, Zusen; He, Lei; Huang, Guanling; Fan, Zusen; Luo, Jianjun; Tian, Yong; Chen, Runsheng
Subjects: Biology >> Biophysics

Hepatocellular carcinoma (HCC) is the most prevalent subtype of liver cancer, and it is characterized by a high rate of recurrence and heterogeneity. Liver cancer stem cells (CSCs) may well contribute to both of these pathological properties, but the mechanisms underlying their self-renewal and maintenance are poorly understood. Here, using transcriptome microarray analysis, we identified a long noncoding RNA (IncRNA) termed IncTCF7 that is highly expressed in HCC tumors and liver CSCs. LncTCF7 is required for liver CSC self-renewal and tumor propagation. Mechanistically, IncTCF7 recruits the SWI/SNF complex to the promoter of TCF7 to regulate its expression, leading to activation of Wnt signaling. Our data suggest that IncTCF7-mediated Wnt signaling primes liver CSC self-renewal and tumor propagation. In sum, therefore, we have identified an IncRNA-based Wnt signaling regulatory circuit that promotes tumorigenic activity in liver cancer stem cells, highlighting the role that IncRNAs can play in tumor growth and propagation.

submitted time 2016-05-11 Hits2081Downloads1317 Comment 0

4. chinaXiv:201605.01239 [pdf]

The Function of lncRNAs in The Caste Determination of The Honeybee

Guo Yu; Zhang Shao-Wu; Chen Run-Sheng; Su Song-Kun; Zhang Shao-Wu; Chen Sheng-Lu; Zhang Shao-Wu; Guo Yu
Subjects: Biology >> Biophysics >> Biochemistry & Molecular Biology

Caste determination in the honeybee has been demonstrated to be decided by the royalactin in the royal jelly. And the differentially expressed coding genes in caste determination of the worker and queen larvae have also been well studied. Our experiments identified that lncRNAs are also differentially expressed in the caste determination of the larvae, which indicates that lncRNAs are involved in the caste determination. Detailed analysis showed that in the early development of the larvae, lncRNAs play a regulatory role in multicellular organismal development, nervous system development and regulation of transcription by affecting the expression and function of the nearby coding genes.

submitted time 2016-05-11 Hits1657Downloads957 Comment 0

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