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1. chinaXiv:202112.00007 [pdf]

AOM DSS诱导炎症引起的小鼠结直肠癌模型尿液蛋白质组的研究

衡姊琦; 高友鹤
Subjects: Biology >> Biochemistry

摘要: [目的]尿液蛋白质组能够灵敏的反映机体的生理变化,可以为炎症引起的结直肠癌的发展提供一定线索。 [方法]本研究使用液相色谱联用质谱(LC-MS/MS),对利用AOM/DSS诱导炎症引起的结直肠癌小鼠模型的尿液差异蛋白进行筛选并进行生物学通路的分析,从生物学通路层面去寻找规律,探究小鼠炎癌模型在结直肠癌的发展中的变化过程。 [结果](1)尿液蛋白质组的变化能够反映实验小鼠的病理生理变化。所有实验小鼠差异蛋白富集到被报道过的且与炎症和肿瘤相关的共有生物学通路有:急性期反应、细胞黏附、天然免疫反应、蛋白激酶B信号的正调控、B细胞活化正调控、糖代谢的相关通路,特别是免疫相关的反应伴随始终。(2)实验小鼠的疾病进展不同,进展不同阶段的小鼠尿液蛋白质组表现不同。本研究运用的单只小鼠独立分析的研究方法能为炎癌模型的小鼠疾病进程提供线索,同时也为未来个性化、精准化疾病分析的方法提供借鉴。

submitted time 2021-12-01 Hits151Downloads69 Comment 0

2. chinaXiv:202111.00034 [pdf]

Revisit of Hsianwenia wui (Cyprinidae: Schizothoracinae) from the Pliocene of Qaidam Basin

BI Dai-Ran; WU Fei-Xiang; WANG Ning; CHANG Mee-Man; FANG Geng-Yu
Subjects: Biology >> Zoology

The Qaidam Basin is a key area for understanding the paleoenvironmental and faunal history of the Tibetan Plateau. The fossil schizothoracine fish, Hsianwenia wui, evolved extraordinarily thickened bones to adapt to the aridification of the Qaidam Basin during the Pliocene. However, the nature of the bone thickening itself remains elusive. To promote the further investigation of the physiological mechanism of the pachyostosis and the phylogenetic interrelationships of Hsianwenia and all relevant cyprinids, here we present a comprehensive morphological study of Hsianwenia. We have new information on the anterior part of the cranial cavity, a large supraneural 3 in the Weberian apparatus, numerous procurrent caudal fin rays supported by the preural centrum (Pu) 5, and a neural arch on Pu2. We also find the differentiated pattern of the bone-thickening: the pachyostosis exists in the endoskeleton but not in the dermal skeleton; it is more obvious in ventral bones than in dorsal ones, when the thickening is present in the dorsally and ventrally grouped endoskeletal bones (e.g., the epineural and epipleural intermuscular bones). Considering the integrity of musculoskeletal system manipulating the chewing activities, we suspect that the thickened pharyngeal jaws and the hard food processing might be associated with the unique hind protrusion (cleithral “humeral” process) of the dermal pectoral girdle of Hsianwenia.

submitted time 2021-11-26 From cooperative journals:《古脊椎动物学报》 Hits302Downloads106 Comment 0

3. chinaXiv:202111.00035 [pdf]

A revision of the eggshell fragment of Spheroolithus megadermus from Laiyang, Shandong Province, China

ZHANG Shu-Kang
Subjects: Biology >> Zoology

In 1958, a special type of dinosaur egg was discovered in Zhaotuan, Laiyang, Shandong Province, China. Although only a large eggshell fragment was collected, its extremely large thickness indicated that the eggshell fragment represented a new ootaxon. In previous studies, it was named Spheroolithus megadermus and assigned to the oogenus Spheroolithus under the oofamily of the Spheroolithidae based on the microstructure in radial view. However, a comparative study of the microstructure in tangential views between the large eggshell fragment from Laiyang and the recently reported Multifissoolithus from Zhejiang Province, China and Yamaguchi, Japan revealed that all of them have roughly paralleled and wavy clefts. Therefore, this study reassigned the large eggshell fragment from Laiyang to Multifissoolithus of the Dongyangoolithidae and discussed its unique compact layer near the eggshell’s inner surface, as well as the chronological and spatial distribution of dongyangoolithid eggs. The reassignment of the holotype of Spheroolithus megadermus also indicates that the referred specimen of Spheroolithus megadermus from Changtu, Liaoning Province becomes the holotype of a new oospecies Spheroolithus quantouensis.

submitted time 2021-11-26 From cooperative journals:《古脊椎动物学报》 Hits228Downloads91 Comment 0

4. chinaXiv:202111.00036 [pdf]

A new aardwolf-line fossil hyena from Middle and Late Miocene deposits of Linxia Basin, Gansu, China

Henry GALIANO; Z. Jack TSENG; Nikos SOLOUNIAS; WANG Xiao-Ming; QIU Zhan-Xiang; Stuart C. WHITE
Subjects: Biology >> Zoology

The aardwolf Proteles cristatus is the only known hyaenid, living or extinct, to exhibit an extremely reduced dentition related to its termite-specializing diet. The fossil record of extant aardwolves extends to 2 to 4 million years ago, but records that inform its evolutionary origins are essentially nonexistent. Such circumstance renders it difficult to place this unusual hyena in the broader evolutionary context of small-bodied hyaenid species in Eurasian Neogene deposits. Here we describe a new genus and species of a small-bodied hyaenid, Gansuyaena megalotis, representing the closest morphological link to aardwolves to date. This new fossil hyena is based on a skull with associated mandible, a rostrum preserving several teeth, and several referred specimens. The new specimens were discovered in Neogene deposits in Linxia Basin, Gansu Province, China. Phylogenetic analysis indicates that among early hyaenids, G. megalotis is most closely related, but unlikely ancestral, to the living aardwolf. Also recognized in this new species are the fossils previously referred to “Protictitherium” aff. P. gaillardi from Pasalar, Turkey. Additionally, “Plioviverrops” guerini from Los Mansuetos, Spain is interpreted to represent a second Gansuyaena species. In addition to the living aardwolf, Proteles cristatus, our analyses suggest that the proteline lineage includes the extinct genera Gansuyaena, Mesoviverrops, and Plioviverrops. Although the precise timing and geographic location of evolutionary divergence between the aardwolf and Gansuyaena remain elusive, critical new morphological information provided by Gansuyaena specimens reinforce findings from recent genomic analyses that the aardwolf lineage has an ancient origin from small-bodied stem hyaenids prior to the appearance of large and robust bone-cracking hyaenines.

submitted time 2021-11-26 From cooperative journals:《古脊椎动物学报》 Hits295Downloads153 Comment 0

5. chinaXiv:202112.00010 [pdf]

中国花楸属单叶类群叶脉序特征研究

田昌芬; 李蒙; 黄亚健; 周源昊; 王贤荣
Subjects: Biology >> Botany >> Applied botany

中国花楸属单叶类群(24 种)包含落萼组与冠萼组,亦有学者将其分为两个属(白花楸属和水榆属)或合并成为一个属(白花楸属)。由于其花序和果实的高度相似性,长期以来分类地位具有争议,而叶脉序特征在花楸属单叶类群中的分类作用尚不明确。因此,该研究以国产花楸属落萼组的?13 个物种和冠萼组的?7 个物种作为研究对象,以清净法制作标本对叶脉序特征进行观测研究,辅助部分叶形态特征进行主成分分析及系统聚类分析。结果表明:(1)中国单叶花楸在叶脉构架上存在一些共性:脉型都为羽状脉、网眼发育情况中等、间二级脉频度在每个粗二级脉间区少于一条;(2)不同物种的粗二级脉构架及对数、有无细二级脉、粗二级脉与主脉夹角的大小、二级脉间三级脉结构、盲脉分支情况、锯齿类型、叶片大小等性状存在一定差异;(3)冠萼组和落萼组组间的脉序特征存在较大的相似性,如粗二级脉构架、叶片大小、盲脉分支情况等多个脉序特征组间存在交叉,基于脉序特征的系统聚类结果不支持冠萼组和落萼组的划分。综上结果可知,叶脉序特征虽然无法作为冠萼组和落萼组的分组依据,但可以为部分单叶花楸的分类提供依据。

submitted time 2021-11-04 From cooperative journals:《广西植物》 Hits93Downloads44 Comment 0

6. chinaXiv:202112.00011 [pdf]

猫须草抗炎活性成分研究

李毅鹏; 潘争红; 符毓夏; 宁德生; 李连春; 海洪
Subjects: Biology >> Botany >> Applied botany

为了研究猫须草(Clerodendranthus spicatus)抗炎活性成分。该研究采用活性追踪的方法,利用硅胶、MCI 柱色谱以及?HPLC 等分离技术,对猫须草抗炎活性部位进行分离纯化,通过波谱数据分析和文献比对鉴定化合物结构;利用?LPS 诱导的?RAW264.7 巨噬细胞炎症模型进行抗炎活性评价。结果表明:(1)从猫须草抗炎活性部位分离得到?10 个化合物,分别鉴定为泡桐素(1)、鼠尾草素(2)、对苯二甲酸二辛酯(3)、N-(N-苯甲酰基-L-苯丙酰基)-L-苯基丙醇(4)、fragransin B1(5)、6,7,8,4'-四甲氧基黄酮(6)、N-反式-阿魏酰酪胺(7)、?N-顺式-阿魏酰酪胺(8)、trans-N-cinnamoyltyramine(9)、新海胆灵A(10),其中化合物?1、?4、8-10 为首次从该植物中分离得到。(2)抗炎结果显示,猫须草抗炎活性成分主要存在于中低极性部位,从中分离得到的大部分化合物显示出一定的?NO 生成抑制活性,其中酰胺类成分(7-9)均具有较好的抗炎活性,表明该类成分是猫须草抗炎作用的主要成分之一。该研究丰富了猫须草抗炎物质基础,为其开发利用提供科学依据。

submitted time 2021-11-04 From cooperative journals:《广西植物》 Hits80Downloads36 Comment 0

7. chinaXiv:202112.00020 [pdf]

广西地不容非药用部位化学成分研究

张汝胜; 李霞; 黄永林; 王亚凤; 阳丙媛; 李典鹏; 何瑞杰
Subjects: Biology >> Botany >> Applied botany

广西地不容(Stephania kwangsiensis)为防己科藤本植物,主要分布于广西,其药用部位(块根)中药名为“金不换”,有镇静、杀虫、抗炎、抗肿瘤、抗病毒、抑菌作用,也用于治疗阿尔茨海默病。为了充分利用该植物资源,明确广西地不容非药用部位中的化学成分,发现相关的活性成分。该研究综合运用正相?Sephadex LH-20、正相硅胶、正相聚酰胺、?MCI gel CHP 20P 等多种柱色谱法对广西地不容非药用部位甲醇提取物进行分离纯化,所得单体化合物的结构经?MS、1H NMR、13C NMR 等波谱学数据的分析以及相关文献的对照进行鉴定,同时用滤纸法对分离得到的化合物开展抗细菌(大肠杆菌、金色葡萄球菌、枯草芽孢杆菌)和抗真菌(尖孢镰刀菌)的活性评价。结果表明:(1)从该植物中分离得到的?10个?化?合?物?分?别?鉴?定?为?吐叶醇?(?1 )、?蚱?蜢?酮?(?2 )、?异?紫?堇?定?碱?(?3 )、?2-O-β-D-glucopyranosyl-D-arabinitol(4)、(9S,12S,13S)-trihydroxyoctadeca-(10E,15Z)-dienoic acid(5)、magnoflorine(6)、corchoionoside C(7)、委陵菜酸(8)、紫堇定碱(9)、克班宁(10)。其中化合物?1、2、4、5、6、7、8 为首次从该植物中分离得到,化合物?1、2、4、?5、7、8 为首次从该属植物中分离得到,化合物?4 为首次从天然产物中分离得到。(2)在所试验的化合物浓度为?6 mg·mL-1时,化合物?1、2、5 对大肠杆菌具有弱抑菌作用;化合物?2对金色葡萄球菌也有弱的抑菌活性;化合物?3 和?8 对枯草芽孢杆菌有弱的抑制作用。广西地不容非药用部位含丰富的活性成分,具有开发利用的潜力。

submitted time 2021-11-04 From cooperative journals:《广西植物》 Hits32Downloads13 Comment 0

8. chinaXiv:202112.00021 [pdf]

多刺绿绒蒿 WD40 基因家族的鉴定及生物信息学分析

任玉玲; 赵艳; 赵成周; 李萍
Subjects: Biology >> Botany >> Applied botany

WD40 转录因子家族广泛参与调节植物生长、发育、次生代谢物积累和环境适应等过程。为了探究?WD40 家族在多刺绿绒蒿生长、发育和次生代谢物积累以及抗逆方面的作用,本研究基于多刺绿绒蒿全长转录组测序数据,鉴定了所有?WD40 基因,并对这些基因及其编码的蛋白进行了生物信息学分析。结果表明:共鉴定到?19 个?WD40 基因,编码的蛋白均具有?WD40 结构域,氨基酸数目?109~758 aa,分子量为11 830~84 130 Da,预测大多数蛋白定位在细胞核中且都为亲水性蛋白;系统进化树分析表明多刺绿绒蒿与罂粟、博落回亲缘关系较近;WD40 基因启动子区域均存在数量不等的激素或逆境响应元件,表明该家族基因可能参与生长、发育和次生代谢物积累等多种生物学进程的调节;蛋白三级结构显示这些蛋白在进化过程中发生了不同程度的进化。这些结果可为深入研究多刺绿绒蒿?WD40 基因家族在其响应逆境胁迫和次生代谢物积累等方面的具体机制提供前期基础。

submitted time 2021-11-04 From cooperative journals:《广西植物》 Hits31Downloads14 Comment 0

9. chinaXiv:202112.00022 [pdf]

紫堇属模式标本馆藏现状及分析

曹家谅; 王静; 付志玺; 刘艳艳; 李家美
Subjects: Biology >> Botany >> Applied botany

模式标本是发表新种的依据,对稳定物种学名至关重要。紫堇属(Corydalis DC.)隶属于罂粟科(Papaveraceae)荷包牡丹亚科(Fumarioideae)紫堇族(Corydaleae),是分类学研究的困难属之一。该研究首先基于《中国植物志》、Flora of China以及全球物种名录查询获得被认可的526个紫堇属物种名录,然后通过检索全球数字化植物标本数据库、全球生物多样性信息网络数据库和中国数字植物标本馆获得了其中395个物种1 894份模式标本,最后对获得的标本信息进行核实、整理和归纳,提取模式类型、馆藏地、采集人、模式产地和采集时间等关键信息进行统计分析。结果表明:紫堇属每个物种平均仅有约5份模式标本;该属模式标本散落在全球80个标本馆中,其中约85%为国外采集者采集。未来应当结合文献,加强对该属模式标本的信息考证和规范化整理。

submitted time 2021-11-04 From cooperative journals:《广西植物》 Hits29Downloads11 Comment 0

10. chinaXiv:202112.00023 [pdf]

红树木榄生境中可培养细菌物种多样性及其体外 抗乙肝病毒活性研究

候师师; 梁考云; 高程海; 蒋翠萍; 唐倩倩; 刘永宏; 易湘茜
Subjects: Biology >> Botany >> Applied botany

红树生境微生物菌群丰富,其次生代谢产物结构新颖,是挖掘新型药物的重要来源。该研究初步评价木榄沉积物、根、叶以及胚轴的可培养细菌多样性以及细菌生物活性,寻找抗乙肝病毒的药源菌株。利用纯培养技术和?16S rRNA 分子生物学技术确定细菌种属并进行物种多样性分析。以?HepG2.2.15 细胞株为模型,通过?MTT 和?PCR 技术测试细菌代谢产物的抗乙肝病毒活性。使用?LC-HRMS 技术对活性菌株代谢产物进行初步分析。结果表明:(1)本次研究共获得细菌?59 种,分属?4 门?5 纲?14 目?23 科?36 属,其中芽孢杆菌为优势菌属。?菌株?GXIMD07402 、?GXIMD07665 、?GXIMD07384 分别为Pseudooceanicola 属、Thioclava 属和?Aestuariibaculum 属潜在新种。(2)抗乙肝病毒活性结果显示?GXIMD07366、GXIMD07616、GXIMD07384 、GXIMD07550、GXIMD07445X提取物能显著降低?HepG2.2.15 细胞上清?HBV DNA 水平(P<0.05),抑制率分别为?51%、?47%、63%、52%、47%。(3)初步鉴定强活性菌株?GXIMD07384 的?4 个主要代谢产物有?Adenosine 、?Cyclo(L-Pro-L-OMet) 、?Acremine G 和?7,8-dimethylbenzo[g] pteridine-2,4(1H,3H)-dione。研究结果表明木榄生境中可培养细菌物种多样性丰富,且含有能产生抗乙肝病毒活性化合物的菌株,为后续海洋微生物资源的应用提供了基础。

submitted time 2021-11-04 From cooperative journals:《广西植物》 Hits26Downloads11 Comment 0

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