Current Location:home > Browse

Institution

Your conditions: Medicine, Pharmacy(1,157)

1. chinaXiv:202203.00025 [pdf]

利用尿液蛋白质组学对阿托伐他汀进行药 物一致性评价

赵晨阳; 陈昱臻; 付晓越; 侯芙芳; 高友鹤
Subjects: Biology >> Biochemistry

[目的]  通过尿液蛋白质组学分析对阿托伐他汀片进行药物一致性评价。

[方法]  分别建立两种药厂的阿托伐他汀片大鼠灌胃模型,收集灌胃前后的尿液,并使用液相色谱串联质谱技术(LC-MS/MS)对提取的尿蛋白进行分析,并通过DAVID数据库对差异蛋白进行生物学通路的分析。

[结果]  灌胃后和灌胃前作比较,在药物A组灌胃模型中共鉴定到116个差异蛋白,在药物B组灌胃模型中共鉴定到66个差异蛋白,其中有24个蛋白被共同鉴定到。这些差异蛋白倾向于参与细胞黏附、内肽酶活性的负调控、蛋白水解等生物学通路。

[结论]  针对产自不同药厂的阿托伐他汀片,我们可以通过尿蛋白检测到它们之间的差别,这验证了尿蛋白的灵敏性,同时提示着尿液蛋白质组学在药物一致性评价上有很大潜力。

submitted time 2022-03-09 Hits5324Downloads816 Comment 0

2. chinaXiv:202203.00009 [pdf]

Effect of High Intensity Interval Training on Blood Glucose Control in Experimental Animal of Type 2 Diabetes Mellitus

谭军
Subjects: Medicine, Pharmacy >> Preclinical Medicine

?Objective?To explore the effect of high intensity interval training (HIIT) on blood glucose control in experimental animal of type 2 diabetes mellitus (T2DM) and to evaluate the quality of the included studies.?Methods?literatures were obtained?by searching PubMed, EMbase, Web of Science, BIOSIS Preview and Cochrane Library. Chinese literatures?were obtained through CNKI, WanFang Data, SinoMed and VIP Database; The collection and screening studies were conducted in strict accordance with inclusion/exclusion criteria. The methodological quality and reporting quality of the included studies were evaluated using SYRCLE's risk of bias tool for animal studies?(SYRCLE) and Animal Research: Reporting In Vivo Experiments guidelines 2.0(ARRIVE2.0). Outcomes,such as body weight (BW), fasting blood glucose (FBG), fasting insulin (FINS), homeostasis model assessment of insulin resistance?(HOMA)?were meta-analyzed and evaluated by Grading of Recommendations Assessment, Development and Evaluation (GRADE).?Results?Twenty studies were included, including male mice (9) or rats (11).?T2DM models have been developed mainly in two ways: high-fat diet and/or combined with streptozotocin (STZ) injection (15 articles), genetic modification (5 articles). Exercise and interval intensity are measured using VO2 max, maximum speed and slope, with 1-4.5 minutes of exercise and 15 second -3 minutes of interval, and the training duration was 8-13 weeks with 3-5 times of training a week.?The SYRCLE’s result?was mainly uncertain;None of the single items or the single studies assessed by?ARRIVE 2.0 appeared complete. Standardized mean difference (SMD) combined the effect size of outcomes showed that compared with the sedentary control group, the heterogeneity was all large, and there?are?significant differences in FBG, HOMA.?Very low quality of all evidence assessed by GRADE.?Conclusions?The effect of HIIT on blood glucose control is clear, but the influencing factors are complex, the risk of bias is high, the quality of reporting is low, the source of heterogeneity in outcomes is complex, and the quality of evidence is extremely low, which make its reliability and validity are questioned. It is recommended to use ARRIVE2.0 as a guide to increase the integrity and transparency of research information and improve research quality through experimental protocol registration and attachments.

submitted time 2022-03-02 Hits3998Downloads473 Comment 0

3. chinaXiv:202111.00037 [pdf]

麻醉分级在围术期死亡风险评估中的作用

刘羡 ; 曹娟; 孙蓓; 蔡宏伟
Subjects: Medicine, Pharmacy >> Clinical Medicine

目的 围术期死亡患者的危险因素分析和麻醉分级在围术期死亡风险评估中的作用。方法 从2007年1月至2010年12月,中南大学湘雅医院共有106,630例手术,其中有214例术后死亡病例。应用结构化问卷,收集患者基本信息和危险因素,采用Cox模型多因素分析筛选围术期死亡的危险因素。结果 214例死亡病例在ASA分级中的分布,每级病例数并没有随等级的增加而增加;而在麻醉分级的分布中,随着等级的增加,各级的数量也随之增加。多因素分析显示,创伤患者的死亡风险高59%;有心血管风险事件(CRE)患者的死亡风险高38%;麻醉分级每增加一级,围术期死亡风险增加45%;心功能分级每增加一级,死亡风险增加41%。结论 创伤、麻醉分级、心功能分级和CRE与围术期死亡相关。麻醉分级每增加一级,围术期死亡危险性增加45%,麻醉分级作为围术期死亡风险评估标准有良好的代表性。

submitted time 2021-11-26 Hits7370Downloads591 Comment 0

4. chinaXiv:202105.00003 [pdf]

SEPRES: Sepsis prediction via a clinical data integration system and real-world studies in the intensive care unit

Chen, Qiyu; Li, Ranran; Lin, Chihche; Lai, Chiming; Chen, Dechang; Qu, Hongping; Huang, Yaling; Lu, Wenlian; Tang, Yaoqing; Li, Lei
Subjects: Medicine, Pharmacy >> Clinical Medicine

Background: Sepsis is vital in critical care medicine, and early detection and intervention are key to survival. We aimed to establish an early warning system for sepsis based on a data integration system that can be implemented in the intensive care unit (ICU). Methods: We trained the LightGBM and multilayer perceptron on the open-source database Medical Information Mart for Intensive Care for sepsis prediction. An ensemble sepsis prediction model was established based on the transfer learning and ensemble learning technique on the private dataset of Ruijin Hospital. The Shapley Additive Explanations analysis was applied to present feature importance on the prediction inference. With the development of data-integrating hub to collect and transmit data from different brands of ICU medical devices, the data integration system was established to receive, integrate, standardize, and store the real-time clinical data. In this way, the sepsis prediction model developed in the ICU of the Ruijin Hospital for the real-world study of sepsis early warning on ICU management. The trial was registered with ClinicalTrials.gov (NCT05088850). Findings: Our best early warning model achieved an area under the receiver operating characteristic curve (AUC) of 0·9833 in the task of detecting sepsis in 4-h preceding on the open-source database, while our ensemble model achieved an AUC of 0·9065?0·9436 in the retrospective research from 1?5-h preceding on the private database, and 0·8636?0·8992 in real-time real-world studies using the data integration system in the ICU of the Ruijin Hospital. In the continuous early warning process of patients admitted to the ICU, 22 patients who met the diagnostic criteria for sepsis during hospitalization were predicted as positive cases; 29 patients without sepsis were predicted as negative cases. Additionally, 17 patients were predicted as false-positive cases; in six patients with sepsis during ICU stay, the predicted probabilities at different time nodes were all less than the warning threshold 0·7 and predicted as false-negative cases. Interpretation: Machine learning models could allow accurate and real-time inference to detect sepsis onset within 5-h preceding at most with the help of the data integration system. We identified the features such as age, antibiotics, ventilation, and net balance to be important for the sepsis prediction inference. We argue that this system has promising potential to improve ICU management by helping medical practitioners identify at-sepsis-risk patients and prepare for timely diagnosis and intervention. Funding: Shanghai Municipal Science and Technology Major Project, the ZHANGJIANG LAB, and the Science and Technology Commission of Shanghai Municipality.

submitted time 2021-11-22 Hits15227Downloads1129 Comment 0

5. chinaXiv:202110.00002 [pdf]

Transplantation of a Beating Heart: A First in Human

Yin Shengli; Rong Jian; Chen Yinghua; Cao Lu; Liu Yunqi; Mo Shaoyan; Li Hanzhao; Jiang Nan; Xiong Mai; Shi Han; Wang Tielong; Shi Yongxu; Zhu Yanlin; Liang Mengya; Xiong Wei; Chen Yili; Xu Guixing; Chen Xiaoxiang; Chen Xiaojun; Yin Meixian
Subjects: Medicine, Pharmacy >> Clinical Medicine

Ischemia and reperfusion injury (IRI), an inevitable component in organ transplantation, contributes to inferior graft survival, compromised organ quality and limitations in organ availability. The heart is most vulnerable to IRI and tolerated ischemic times are very short. We present an entire novel heart transplant procedure during which the donor heart was continuously perfused, oxygenated at normothermia and transplanted while beating. The dynamic electrocardiogram and echocardiogram indicated an absence of ischemic injury and excellent function of the donor heart during the entire procedure. This ischemia-free beating heart transplantation (IFBHT) avoids graft IRI. The approach has thus the potential to improve transplant outcome while improving organ utilization.

submitted time 2021-10-02 Hits11626Downloads920 Comment 0

6. chinaXiv:202108.00058 [pdf]

Metagenomic evidence for the coexistence of SARS and H1N1 in patients from 2007-2012 flu seasons

Qi Liu; Zhenglin Du; Sihui Zhu; Wenming Zhao; Hua Chen; Yongbiao Xue
Subjects: Biology >> Microbiology

By re-analzying public metagenomic data from 101 patients infected with influenza A virus during the 2007-2012 H1N1 flu seasons in France, we identified 22 samples with SARS-CoV sequences. In 3 of them, the SARS genome sequences could be fully assembled out of each. These sequences are highly similar (99.99% and 99.7%) to the artificially constructed recombinant 5 SARS-CoV (SARSr-CoV) strains generated by the J. Craig Venter Institute in USA. Moreover, samples from different flu seasons have different SARS-CoV strains, and the divergence between these strains cannot be explained by natural evolution. Our study also shows that retrospective studies using public metagenomic data from past major epidemic outbreaks serves as a genomic strategy for the research of origins or spread of infectious diseases.

submitted time 2021-08-11 Hits17057Downloads1776 Comment 0

7. chinaXiv:202107.00039 [pdf]

Tracing the origin of SARS-CoV-2: lessons learned from the past

Qihui Wang; Hua Chen; Yi Shi; Alice C. Hughes; William J. Liu; Jingkun Jiang; George F. Gao; Yongbiao Xue; Yigang Tong
Subjects: Biology >> Virology

新冠病毒的起源仍不清楚。了解新冠病毒如何、何时以及在何处从其天然宿主传播给人类对于预防未来由冠状病毒引发的疫情至关重要。 从与病原体无休止的战斗中吸取教训,结合目前已知的关于新冠病毒起源和中间宿主的研究数据,我们提出全球多个地点都有可能是新冠病毒的起源地。

submitted time 2021-07-28 Hits18383Downloads2075 Comment 0

8. chinaXiv:202107.00013 [pdf]

On the origin of SARS-CoV-2–The blind watchmaker argument

Chung-I Wu; Haijun Wen; Jian Lu; Xiao-dong Su; Alice C. Hughes; Weiwei Zhai; Chen Chen; Hua Chen; Mingkun Li; Shuhui Song; Zhaohui Qian; Qihui Wang; Bingjie Chen; Zixiao Guo; Yongsen Ruan; Xuemei Lu; Fuwen Wei; Li Jin; Le Kang; Yongbiao Xue
Subjects: Biology >> Virology

In the comparison with SARS-CoV of 2003, SARS-CoV-2 is extremely well adapted to the human populations and its adaptive shift from the animal host to humans must have been even more extensive. By the blind watchmaker argument, such an adaptive shift can only happen prior to the onset of the current pandemic and with the aid of step-by-step selection. In this view, SARS-CoV-2 could not have possibly evolved in an animal market in a big city and even less likely in a laboratory. Discussions of the origin of SARS-CoV-2 need to factor in the long process of adaptive shift and some models have indeed advanced in that direction.

submitted time 2021-07-17 Hits15579Downloads1523 Comment 0

9. chinaXiv:202107.00008 [pdf]

GT198蛋白是抗癌化药和抗癌草药的靶点

庞峻峰; 高洁; 张立勇; Nahid F. Mivechi ; 柯蓝
Subjects: Medicine, Pharmacy >> Pharmacology

肿瘤血管生成是癌症的一个特征。一些抗血管生成的抗癌药物已被证明临床有疗效。我们之前的研究显示,在包括口腔癌在内的人类实体瘤中,GT198 蛋白(基因符号PSMC3IP,又称为Hop2)是一个诱导血管生成的癌蛋白。本研究结果表明,十几种临床抗癌化疗药物,和几种临床成功的抗癌草药,都能直接抑制GT198蛋白靶点。GT198是一个能与DNA结合的DNA修复蛋白。利用体外DNA结合法检测GT198活性,我们测试了129个美国NCI收集的临床抗癌化疗药物。发现体外直接抑制GT198的化药包括但不限于米托蒽醌、多柔比星(阿霉素)、紫杉醇、依托泊苷、放线菌素D和伊马替尼(格列卫)。紫杉醇和依托泊苷具有较高的结合亲和力,而多柔比星由于竞争性抑制GT198,具有较高的结合效力。由于GT198与DNA拓扑异构酶有蛋白序列同源性,而DNA拓扑异构酶是过去认为的药物靶点,因此,GT198蛋白很可能是个以前未被认知的药物新靶点。为了寻求更高效的GT198抑制剂,我们进一步测试了几种抗癌草药的提取物。发现具有高亲和力和高效力的阳性抑制草药都是临床上已经有成功历史的抗癌草药,包括牙买加的多香果(Allspice)、中国的皂角刺(Gleditsia sinensis L.),和厄瓜多尔的BIRM。我们使用有机化学方法对多香果进行了部分纯化,证明用GT198为靶标,以体外DNA结合法追踪测活,可以高效快速纯化天然产物。综上表明,此项研究揭示了GT198是多种抗癌化药的新靶点。这项研究还提供了一个鉴定化合物与纯化天然产物的优异药物靶标。特别关键的是,这项研究为快速分离高效低毒的抗癌中草药提供了绝佳机会。

submitted time 2021-07-12 Hits15277Downloads1121 Comment 0

10. chinaXiv:202106.00110 [pdf]

利用一对多尿液蛋白质组比较为发热待查提供线索

赵晨阳; 魏利龙; 魏静; 高友鹤
Subjects: Biology >> Biochemistry

发热待查是指不明原因的发热,尽管医疗诊断技术在不断发展,但是发热待查仍旧是亟待解决的难题。尿液是机体排泄代谢废物的一种途径,由于不受到稳态调节机制的影响,尿液能反映机体产生的微小变化,比血液更早期、更敏感。在本研究中,我们对收集的尿液样本采用一对多的尿液蛋白质组比较方法,即一个病人和一组健康人样本对比分析的方法,旨在通过差异蛋白来寻找与疾病相关的生物学通路,为发热待查的临床诊断提供线索和依据。分析结果表明,差异蛋白富集到的生物学通路与发热有关,尿蛋白质组能够提供病人和健康人的区别;同时一对多的研究方法能为发热待查患者提供个性化的线索,也是未来探索病人未知疾病的一个方法。

submitted time 2021-06-25 Hits15312Downloads1449 Comment 0

12345678910  Last  Go  [116 Pages/ 1157 Totals]