Subjects: Biology >> Botany >> Applied botany submitted time 2021-12-19 Cooperative journals: 《广西植物》
Abstract： In order to explore the response mechanism of AP2/ERF gene family in the water stress of Olea europaea, this study performed transcriptome sequencing on the roots and leaves of two cultivars 'Frantoio' and 'TYZ-1' that were under drought and flooding stress. And based on the whole genome data, the protein physicochemical properties, gene structure and system evolution of AP2/ERF transcription factor in O. europaea were analyzed. At the same time, the difference in gene expression of AP2/ERF transcription factor related to water stress in the two O. europaea cultivars was analyzed by transcriptome sequencing data and verified by fluorescence quantitative PCR. The results were as follows: (1) A total of 110 AP2/ERF gene family members were identified in O. europaea. The amino acid size of the 110 proteins is 173-717bp, there is no signal peptide and it is a non-secreted protein. The phylogenetic tree was constructed between O. europaea AP2/ERF and model plant Arabidopsis AP2/ERF protein. It was found that O. europaea AP2/ERF protein was divided into four categories: AP2, RAV, ERF and Solosist. Among them, ERF is divided into two subtypes: ERF and DREB. ERF includes six subtypes of ERF B1 to ERF B6, and DREB includes six subtypes of DREB A1 to DREB A6, which is consistent with the classification of the model plant Arabidopsis AP2/ERF. Each subfamily contains AP2/ERF proteins of O. europaea and Arabidopsis at the same time, indicating that the AP2/ERF family of Arabidopsis and O. europaea are similar in evolution. (2) The analysis of gene structure and conserved elements found that the proteins of the same subfamily of O. europaea AP2/ERF have the same gene structure and conserved elements. Combining gene expression with genes with known water regulation functions in the evolutionary tree, it is preliminarily speculated that OeAP2-75, OeAP2-97, OeAP2-101, OeAP2-23, OeAP2-13 are closely related to the water regulation of O. europaea, OeAP2-13, OeAP2-28, OeAP2-104, OeAP2-75, OeAP2-80, OeAP2-50 have different expression levels in the two varieties. It is speculated that this may be the reason for the different resistance of 'Frantoio' and 'TYZ-1'. The RT-qPCR technique was used to detect the expression changes of O. europaea AP2/ERF gene under different stresses. The results showed that OeAP2-101, OeAP2-28, and OeAP2-42 were significantly up-regulated by water stress, which was consistent with the results of transcriptome analysis. The results of this study can lay a foundation for the research on the stress resistance expression and gene function of the AP2/ERF family genes of O. europaea, and provide a method and theoretical basis for the selection of drought-resistant and flood-tolerant rootstock varieties of O. europaea.
Subjects: Biology >> Botany >> Applied botany submitted time 2019-10-17 Cooperative journals: 《广西植物》
Abstract：为了解降香黄檀叶绿体基因组密码子使用模式，本研究利用Codon W 1.4.2和在线软件CUSP对降香黄檀叶绿体基因组中的52条基因编码序列密码子进行中性绘图、ENC-plot和PR2-plot分析。结果表明：降香黄檀叶绿体基因组密码子的3个位置上GC含量依次为GC1 (46.01%) >GC2 (38.98%) >GC3 (27.80%)；有效密码子数(ENC)范围为37.66 ~ 54.43，及ENC值>45的有37个；RSCU >1的密码子有29个，其中16个以U结尾、12个以A结尾；这些说明其偏好以AT结尾，且偏性较弱。中性绘图分析显示GC12与GC3的相关系数0.250，相关性不显著，回归系数为0.394；ENC-plot分析显示ENC比值位于-0.05 ~ 0.15区间的基因有39个；且PR2-plot分析在碱基的使用频率方面，U > A、G > C；说明降香黄檀叶绿体基因组密码子偏好性主要受选择的影响；19个密码子被确定最优密码子。本研究为降香黄檀叶绿体基因工程、遗传多样性分析等研究提供了科学参考依据。
Subjects: Biology >> Botany >> Applied botany submitted time 2019-02-25 Cooperative journals: 《广西植物》
Abstract：由氨基酸衍生的氰苷是由植物细胞色素 P450 单加氧酶(CYP)催化氨基酸生成的次生代谢产物，与植物的防御和抗逆境胁迫相关。本研究通过分析蒜头果转录组数据，从中分离并克隆得到 1 条细胞色素 P450 基因(命名为 MoCYP71，GenBank 登录号 MK172858)，并对其进行生物信息学分析及检测该基因在果实不同发育时期的表达情况。结果显示，MoCYP71基因含 1 572 bp，编码 523 个氨基酸，该基因的 cDNA 序列与咖啡(Coffea eugenioides,XM_027319282)、小果咖啡(Coffea arabica，XM_027213456)中的 CYP71 基因的 mRNA 序列均有 88 %的一致性 ；该蛋白的相对分子质量为 58 976.54，理论等电点 pI 为 8.10，分子式为 C2675H4184N704O744S27，不稳定系数(Ⅱ)为 40.84，是一种不稳定蛋白；该蛋白不存在信号肽，存在于分泌途径，含有两个跨膜结构，分别位于 20~37 和 311~333 位氨基酸为跨膜疏水螺旋，锚定于细胞器上；MoCYP71 蛋白含有 CYP 家族的保守结构域，包括脯氨酸富集区(PPSPPRLP)、K 螺旋(KETFR)、I 螺旋(GGIDTS)、PERF 域(PERF)和识别结构域血红素结合域(FGAGRRICPG)，与可可、榴莲、高粱等的 CYP71E 家族的蛋白(GenBank 登录号分别为 EOX92908.1、XP_022773875.1 和 AAC39318.1)聚为一支；在花谢后，MoCYP71 基因表达量逐渐降低， 花谢后 1 个月>2 个月>3 个月，但在花谢后 4 个月的表达量急剧增加。本研究对研究蒜头果的对虫害的防御、组织成熟及蒜头果中有效次生代谢产物的发掘具有重要的意义。
Subjects: Biology >> Botany submitted time 2018-03-29 Cooperative journals: 《广西植物》
Abstract：蒜头果是我国特有的单种属稀有树种。本研究对来自蒜头果的植物内生真菌（白黄笋顶孢霉、哈茨木霉、大棘黑团孢、枝状枝孢菌、斑污拟盘多毛孢、赭绿青霉、淡紫紫孢菌、朱黄青霉、Xenoacremonium recifei、Xylaria feejeensis）进行液体培养，10 d后回收培养液并用乙酸乙酯萃取获得初提物，采用抑菌圈法检测蒜头果内生真菌初提物抑菌活性，同时测定了最低抑菌浓度（MIC）。结果表明：白黄笋顶孢霉、大棘黑团孢、枝状枝孢菌、斑污拟盘多毛孢、赭绿青霉、淡紫紫孢菌均有抑菌活性，大棘黑团孢、斑污拟盘多毛孢、淡紫紫孢菌的初提物均对缓慢芽孢杆菌、无乳链球菌和藤黄微球菌有明显抗菌活性，最低抑菌浓度在1.5625 ~ 6.25 mg·mL-1之间。研究表明蒜头果树皮内生真菌的次生代谢产物具有抗菌活性，各内生真菌次生代谢产物的抗菌效果不同。该研究结果为蒜头果树皮内生真菌的抗菌活性化合物的开发利用奠定了基础。
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