Subjects: Agriculture, Forestry,Livestock & Aquatic Products Science >> Plant Protection submitted time 2018-06-22 Cooperative journals: 《广西植物》
Abstract: Oilseeds of camphor tree (Cinnamomum camphora (L.) Presl.) contains high content of medium chain fatty-acyl (MCFA). However, the biosynthetic mechanism is still missing. Long chain fatty aycl-coenzyme (CoA) synthetases (LACSs) can catalyze free fatty acids to acyl-CoA thioesters and play important roles in fatty acid metabolism in terrestrial plant. In this study, the cDNA encoding AtLACS9 homologous was identified and cloned based on transcriptomes data of C.camphora, sharing 75% sequence similarity to AtLACS9 and being registered to CcLACS9. Multiple sequences alignment shows CcLACS9 possesses three plant LACS-specific motifs and plastidical targeting signal in N-terminal. Using oleic acid as a substrate, CcLACS9 could activate free fatty acids into acyl-CoA thioesters in a yeast mutant deficient in LACS complementation test.