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A facile method for studying interaction of rhodamine B and bovine serum albumin: Towards physical-binding mediated fluorescence labeling of proteins postprint

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Abstract: Strategies for labeling proteins with fluorophores are always important for biotechnology. Here we take a model protein (bovine serum albumin) and a typical fluorophore (rhodamine B) to demonstrate a direct labeling method just by physical adsorption. In combination with size exclusion chromatography and the Scartchard equation, we have developed a facile analysis method for calculating the binding constant and binding sites. The molecular docking method has been used to study the binding site in amino acid level.

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[V1] 2023-06-18 18:11:13 ChinaXiv:202306.00209V1 Download
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