Subjects: Digital Publishing >> New Media submitted time 2023-10-08 Cooperative journals: 《中国传媒科技》
Abstract：随着经济的蓬勃发展,新媒体凭借着立体化传播、互动性好、容量大、时效性强等优势,给高校校报带来了巨大的挑战,直接冲击了其在校园的主流媒体地位。因此,为了顺应时代的发展趋势,高校校报必须重新找准自身定位,分析自己的优劣势,找到适合高校校报的创新转型之路,从而实现新的发展与突破,对其主流媒体地位进行巩固与加强,为我国高校校报的长远发展打下坚实的基础。
Subjects: Biology >> Bioengineering submitted time 2018-07-15 Cooperative journals: 《中国生物工程杂志》
Abstract：目的：建立钙通道Orai1的体外研究方法。方法：利用脂质体重组技术，将体外纯化的Orai1蛋白重组到脂质体膜上，并利用蔗糖密度梯度离心来检测其重组效率及Orai1蛋白在脂质体膜上的结构，并利用钙染料Fura-2检测脂质体内钙离子的释放。结果：成功制备了脂质体及体外纯化了GST-Orai1融合蛋白，蔗糖密度梯度离心结果证明GST-Orai1蛋白成功重组到脂质体上，以及Orai1蛋白以多聚体的形式定位在脂质体膜上。钙离子释放实验证明脂质体内钙离子包装完好，可用于后续Orai1钙通道的功能研究。结论：利用脂质体重组技术建立了一种新的Orai1的研究方法，能够更直接有效地研究其功能及其活化机制。
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2018-01-25 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To construct the KbvR gene of LuxR family deletion mutant and complementation strains from Klebsiella pneumoniae NTUH-K2044 and analyze the effect of KbvR on bacterial growth, biofilm formation and capsular synthesis. Methods A KbvR gene deletion mutant strain was constructed using the suicide vector pKO3-Km, and the gene fragment including KbvR coding region,promoter area and transcription termination area were amplified and cloned into pGEM-T-easy plasmid to construct KbvR complementation strain. The growth curves of the wild-type strain, KbvR gene deletion mutant strain and complementation strain were observed to assess the effect of KbvR on bacterial growth. Crystal violet staining method was used to measure the effect of KbvR on biofilm formation; the effect of KbvR on capsular synthesis was detected using string test, centrifugal test and RT-PCR. Results The KbvR deletion mutant and complementation strains were constructed successfully. KbvR gene did not affect the growth of the bacteria, but biofilm formation and capsular synthesis were attenuated in KbvR deletion mutant strain. Conclusion As a transcription factor of the LuxR family orphans of the quorumsensingsystem,KbvRpositivelyregulatesbacterialbiofilmformationbyaffectingcapsularsynthesis.
Subjects: Nursing >> Nursing submitted time 2025-07-17
Abstract： [Objective] To translate and adapt the Attitude Scale Towards the Use of Artificial Intelligence Technologies in Nursing (ASUAITIN) into Chinese, and to test its reliability and validity, so as to provide an evaluation tool for assessing the attitudes of clinical nurses towards the application of artificial intelligence technologies in their work. [Methods] Using the Brislin translation model, the scale was translated, back-translated, culturally adapted, and pre-investigated to form the Chinese version of ASUAITIN. By convenience sampling, a questionnaire survey was conducted among 396 nurses in a tertiary first-class hospital in Beijing in March 2025 to test the reliability and validity of the scale. [Results] The Chinese version of ASUAITIN consisted of 15 items in 2 dimensions. The Cronbach’s α coefficient of the scale was 0.939, the split-half reliability was 0.738, the Cronbach’s α coefficients of each dimension were 0.945 and 0.956 respectively, and the test-retest reliability was 0.935. The content validity index at the scale level was 0.981, and the content validity index at the item level ranged from 0.875 to 1.000. Through exploratory factor analysis, 2 common factors were extracted, with a cumulative variance contribution rate of 77.402%. The results of confirmatory factor analysis (CFA) showed that the chi-square to degrees of freedom ratio (χ²/df) was 2.242, the Comparative Fit Index (CFI) was 0.966, the Goodness-of-Fit Index (GFI) was 0.882, the Adjusted Goodness-of-Fit Index (AGFI) was 0.941, the Incremental Fit Index (IFI) was 0.967, the Tucker-Lewis Index (TLI) was 0.959, the Relative Fit Index (RFI) was 0.929, and the Root Mean Square Error of Approximation (RMSEA) was 0.079. [Conclusion] The Chinese version of ASUAITIN has good reliability and validity, and can be used to evaluate the attitudes of Chinese clinical nursing staff towards the application of artificial intelligence technologies.
Subjects: Medicine, Pharmacy >> Preventive Medicine and Hygienics submitted time 2023-07-04 Cooperative journals: 《中国全科医学》
Abstract： Background The prevalence of hypertension in Chinese population is still increasing by year，and the significant increase in the prevalence of chronic diseases caused by aging of the population results in a serious medical burden. There are still about 450 million people in China who use solid fuels for cooking or heating，causing indoor air pollution as an important public health problem in China. At present，several domestic and foreign studies have confirmed the correlation of solid fuels use with the increased risk of hypertension in adults，while the relationship between solid fuels use and the risk ofhypertension in Chinese older adults still remains unclear. Objectives To analyze the association between solid fuels use and the risk of hypertension in older adults in urban and rural areas of China through a prospective cohort study，so as to provide a theoretical basis for the prevention and treatment of hypertension in the elderly. Methods This study is a prospective cohort study. Data were obtained from the 2011 to 2018 China Longitudinal Healthy Longevity Survey（CLHLS）by using baseline questionnaire and physical examination. A total of 1 453 non-hypertensive older adults aged 65 years and above in the 2011 cohort were included in the study，and the occurrence of hypertension during follow-up was considered as the outcome indicator. The included subjects were divided into the clean fuel（electrical appliances such as induction cooker，pipeline natural gas，solar energy）group（n=654）and solid fuel（charcoal，kerosene，coal，firewood）group（n=799）. Multivariate Cox proportional hazard regression model was used to analyze the association between cooking with solid fuels and the risk of hypertension. ResultsA total of 1 453 non-hypertensive older adults at baseline with an average age of（77.6±8.8）years and a median follow-up of 7 years，and a total of 838 people developed hypertension at the outcome. The results of this study showed that the proportion of cooking with solid fuels in rural areas was significantly higher than that in urban areas（70.3% vs.1.1%，P<0.05）. Multivariate Cox proportional hazard regression model showed that cooking with solid fuels significantly increased the risk of hypertension〔HR=1.20，95%CI（1.05，1.38），P=0.01〕，and the adjustment for covariates still indicated that it increased the risk of hypertension〔HR=1.21，95%CI（1.04，1.41），P=0.01〕. In addition，cooking with solid fuels was positively associated with abnormal mean arterial pressure（MAP）〔HR=1.26，95%CI（1.02，1.55），P=0.03〕；and the adjustment for covariates still showed positive association of it with abnormal MAP〔HR=1.28，95%CI（1.02，1.60），P=0.03〕. Conclusion Cooking with solid fuels in urban and rural areas of China significantly increases the risk of hypertension in the elderly，as well as affects the mean arterial pressure. Reducing the use of solid fuels for cooking and promoting the use of clean fuels is a simple and effective measure in the prevention and control of hypertension.
Subjects: Medicine, Pharmacy >> Preventive Medicine and Hygienics submitted time 2023-03-28 Cooperative journals: 《中国全科医学》
Abstract： Objective To evaluate the impact of widowhood on the mental health of older adults in China，and provide a basis and reference for formulating the mental health intervention program for older adults in China. Methods Using the 2018 China Health and Retirement Longitudinal Study （CHARLS） as the data source，4694 older adults aged 60 years and above were selected as study subjects and divided into the widowed group （n=644） and non-widowed group （n=4050）. The mental frailty index was used as a perspective for evaluating the mental health status of older adults. Using propensity score matching（PSM）model，the propensity score was calculated by using Logistic regression analysis，the robustness was tested by using standard deviation variation，common support bar graph and nuclear density distribution，the impact of widowhood on the physical and mental health of older adults was analyzed by using K-nearest neighbor matching，radius matching and kernel matching，and the heterogeneity was tested based on gender，household type and age. Results Compared to the non-widowed group，the mental health of older adults in the widowed group was impaired （P<0.001）. Logistic regression model results showed that gender，age，education level and monthly family consumption were correlated with widowhood（P<0.001）. The robustness test results after matching were better. The results of PSM showed that widowhood significantly increased the mental frailty index level（ATT ranged from 0.054 to 0.055，P<0.001）. The results of heterogeneity test showed that there were significant differences between urban and rural areas and among different age groups in the impact of widowhood on the mental frailty index of older adults，but there was no significant difference between male and female in the impact of widowhood on the mental frailty index of older adults. Among them，the impact of widowhood on the older adults in rural areas was more significant（ATT ranged from 0.072 to 0.075，P<0.001），but the impact on older adults in urban areas was not significant；the impact on older adults in the low age group and high age group was more significant（ATT ranged from 0.061 to 0.081，P<0.01；0.067~0.078，P<0.05），but the impact on older adults in the middle age group was not significant. Conclusion Widowhood has a negative impact on the mental health of the older adults. The impact varies among different older adults. The widowed older adults in rural areas，just entering old age and in their later years need attention and psychosocial support.
Subjects: Psychology >> Physiological Psychology Subjects: Psychology >> Personality Psychology Subjects: Other Disciplines Subjects: Psychology >> Educational Psychology submitted time 2024-10-30
Abstract： The phenomenon of individual differences in emotional responses to music is prominent, manifesting in the diverse emotional experiences elicited by the same piece of music in different individuals. Some people experience rich emotions, while others remain relatively unaffected. Previous research suggests that empathy may be a crucial factor in understanding and influencing these emotional responses to music. However, the psychological processes and outcomes associated with emotional responses to music remain unclear. This gap in knowledge is significant for the study of music psychology, educational psychology, and affective psychology, particularly in understanding the psychological processes underlying emotional responses to music and how to enhance these responses in individuals. Therefore, this study aims to investigate the potential behavioral and neural impacts of empathy differences on emotional responses to pipa music using functional near-infrared spectroscopy (fNIRS). This study employs a naturalistic passive listening paradigm. In the preliminary phase of the experiment, the Chinese version of the Interpersonal Reactivity Index (IRI-C), the Generalized Anxiety Disorder Scale (GAD-7), and the Patient Health Questionnaire-9 (PHQ-9) were distributed online to recruit university students. Based on responses from 402 questionnaires, 49 university students with high and low levels of empathy were selected to participate in the near-infrared spectroscopy (fNIRS) experiment. The experiment utilized a 2 (Empathy: high, low) x 2 (Musical Emotional Valence: positive, negative) mixed design. All audio recordings were performed by a professional pipa teacher, with segments selected from the "Pipa Music Scores - An Anthology of Chinese Ethnic Instrumental Music." After evaluating the emotional valence of the materials, seven segments each of positive and negative emotional valence were chosen as experimental materials. Following the music playback, emotional responses were measured using both a three-dimensional model of emotion (valence, energy arousal, tension) and categorical models(6 basic emotions, 9 aesthetic emotions). Concurrently, functional near-infrared spectroscopy (fNIRS) was used to record the concentration of oxyhemoglobin (HbO) across 81 channels of the whole brain. The behavioral results indicate that the high-empathy group exhibited stronger emotional arousal and elicited more basic and aesthetic emotions compared to the low-empathy group. The fNIRS results revealed that, in comparison to the low-empathy group, the high-empathy group showed greater activation in the pre-motor and supplementary motor cortex and higher-order cognitive processing areas (such as the dorsolateral prefrontal cortex, and frontopolar area). These results suggest that: 1) Empathic differences affect the mental processes of individual musical emotional responses, which are reflected in the differences between bottom-up embodied imitation and top-down cognitive processing. 2) Empathic differences affect the outcomes of individual musical emotional responses, which are reflected in the differences in the richness and intensity of emotions.
Peer Review Status:
Awaiting Review
Subjects: Psychology >> Developmental Psychology Subjects: Psychology >> Educational Psychology submitted time 2020-11-26
Abstract： From the perspective of life course, school transition is considered a key turning point in adolescent development and may alter individual developmental trajectories. Based on the deficit view, previous studies have suggested that most adolescents are at risk, beset by many challenges, and may experience a variety of negative developmental outcomes during that transition. Drawing on the positive youth development perspective, however, the transition period may be an important opportunity for adolescents to achieve adaptive and healthy development, and it is also the period in which developmental plasticity is most fully manifested. Along this line, if adolescents’ internal and external assets are well integrated, they may have a positive school transition." " "
Subjects: Nursing >> Nursing submitted time 2023-03-06
Abstract： Objective? To explore the application effect of simulated teaching based on standardized case database combined with guided feedback method in the practice teaching of nursing undergraduates. Methods Cluster sampling method was adopted to select 216 nursing undergraduates of 2016 in school as the research object. A standardized case database was established, and s simulated teaching combined with guided feedback method was adopted in the practice teaching of nursing undergraduates. The critical thinking and independent learning ability of nursing undergraduates before and after practical teaching were compared. Results After the implementation of scene simulation teaching based on standardized case database combined with guided feedback teaching mode, scores of assessments on critical thinking and independent learning ability of nursing undergraduates were improved, compared with those before intervention, the difference was statistically significant (P＜0.05). Conclusion Scene simulation teaching based on standardized case database combined with guided feedback teaching mode can improve the critical thinking and independent learning ability of nursing undergraduates, which is worthy of promotion and application.
Peer Review Status:Awaiting Review
Subjects: Medicine, Pharmacy >> Preventive Medicine and Hygienics submitted time 2023-06-16 Cooperative journals: 《中国全科医学》
Abstract： Background High serum uric acid（SUA）levels may be associated with hypertriglyceridemia.However，prospective cohort studies on the effect of longitudinal trajectory changes in SUA on new-onset hypertriglyceridemia are still lacking. Objective To investigate the correlation between the longitudinal trajectory of SUA and the new-onset hypertriglyceridemia. Methods A total of 3871 architecture employees who underwent physical examinations in Tiejian Hospital of Shiyan from 2015 to 2020 were selected as study subjects. The general data，physical examination data and laboratory test results of the study subjects were collected. Group-based trajectory model（GBTM）was used to group the uric acid trajectories of the study subjects，linear trend χ2 test was used to detect linear trends in the density of hypertriglyceridemia development with SUA stratification. Generalized estimation equation（GEE）was used to analyze the relationship between each index and hypertriglyceridemia. Results The trajectories of GBTM group 1 showed low SUA fluctuation levels（250~350 μmol/L），group 2 showed medium SUA fluctuation levels（>350~450 μmol/L），and group 3 showed high SUA fluctuation levels（>450 μmol/L），the three groups were named as low fluctuation group（n=1103），medium fluctuation group（n=2141），and high fluctuation group（n=627）sequentially according to the trajectory characteristics. The results of linear trend χ2 test revealed that the density of hypertriglyceridemia development increased with the rise of uric acid fluctuating levels（χ2 =146.728，P<0.001）. There were significant differences in age，total cholesterol（TC），triacylglycerol（TG），SUA，creatinine （Cr），systolic blood pressure（SBP），diastolic blood pressure（DBP），and BMI among the three groups（P<0.05）. GEE analysis showed that TC，Cr，SBP，DBP，fasting blood glucose and BMI were influencing factors in the development of hypertriglyceridemia（P<0.05），and the risk of hypertriglyceridemia was increased in the medium fluctuation group〔RR=2.294，95%CI（1.834，2.868）〕and high fluctuation group〔RR=3.012，95%CI（2.295，3.953）〕using the low fluctuation group as a reference. Conclusion Elevated Longitudinal trajectory of serum uric acid level is a risk factor for new-onset hypertriglyceridemia.
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