A facile method for studying interaction of rhodamine B and bovine serum albumin: Towards physical-binding mediated fluorescence labeling of proteins

分类： 物理学 >> 核物理学 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》

MA Yu-Xing ZHONG Rui-Bo GUO Jun LIU Yu-Shuang YUAN Ming BAI Zhi-Jun LIU Tao-Tao ZHAO Xin-Min ZHANG Feng

摘要： Strategies for labeling proteins with fluorophores are always important for biotechnology. Here we take a model protein (bovine serum albumin) and a typical fluorophore (rhodamine B) to demonstrate a direct labeling method just by physical adsorption. In combination with size exclusion chromatography and the Scartchard equation, we have developed a facile analysis method for calculating the binding constant and binding sites. The molecular docking method has been used to study the binding site in amino acid level.

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