分类： 生物学 >> 生物物理学 >> 细胞生物学 提交时间： 2016-05-12
摘要： Super-resolution microscopy techniques have overcome the limit of optical diffraction. Recently, the Bayesian analysis of Bleaching and Blinking data (3B) method has emerged as an important tool to obtain super-resolution fluorescence images. 3B uses the change in information caused by adding or removing fluorophores in the cell to fit the data. When adding a new fluorophore, 3B selects a random initial position, optimizes this position and then determines its reliability. However, the fluorophores are not evenly distributed in the entire image region, and the fluorescence intensity at a given position positively correlates with the probability of observing a fluorophore at this position. In this paper, we present a Bayesian analysis of Bleaching and Blinking microscopy method based on fluorescence intensity distribution (FID3B). We utilize the intensity distribution to select more reliable positions as the initial positions of fluorophores. This approach can improve the reconstruction results and significantly reduce the computational time. We validate the performance of our method using both simulated data and experimental data from cellular structures. The results confirm the effectiveness of our method.
分类： 化学 >> 物理化学 提交时间： 2017-11-05 合作期刊: 《结构化学》
摘要： Two coordination polymers (CPs), {[Ag(ADA)0.5(DPE)]·H2O}n (1) and {[Ag(ADA)0.5(Bipy)0.5]·H2O}n (2), (H2ADA = 3,3-azodibenzoic acid, DPE = 1,2-di(4-pyridyl)- ethylene, Bipy = 4,4΄-bipyridine), have been synthesized via solvothermal reactions of Ag+ with ADA2- and/or DPE, Bipy. CPs 1 and 2 were characterized by elemental analyses, IR spectra, thermal behaviors, and X-ray single-crystal diffraction. CPs 1 and 2 feature “Ag···Ag” subunits. CP 1 shows a 3D construction, in which the “Ag···Ag” subunits extend through ADA2- and DPE ligands using μ6-kO,O;kO;kO;kO,O;kO;kO and μ2-kO;kO coordination modes, respectively. It crystallizes in triclinic system, space group P, with a = 0.3808(18), b = 1.2476(6), c = 1.309(6) nm, α = 76.757(5), β = 84.649(5), γ = 86.809(5)º, V = 0.6024(5) nm3, Z = 2, Mr = 351.11, C13H11AgN2O3, Dc = 1.936 g/cm3, F(000) = 348, S = 1.064, R = 0.0323 and wR = 0.0689. CP 2 exhibits a 3D supramolecular structure, in which the “Ag···Ag” subunits are connected by ADA2- and DPE ligands with μ6-kO;kO;kO,O;kO;kO and μ2-kO;kO coordination modes, respectively. Ultimately, through hydrogen bond and π···π interaction, the “Ag···Ag” subunits stabilize the 3D supramolecular structure of 2. It crystallizes in monoclinic, space group C2/c, with a = 25.301(15), b = 13.197(8), c = 6.970(4) nm, β = 102.597(7)º, V = 2.271(2) nm3, Z = 8, C12H10AgN2O2.50, Mr = 330.09, Dc = 1.931 g/cm3, F(000) = 1304, S = 1.082, R = 0.1107 and wR = 0.2984. The fluorescence properties of CPs 1 and 2 have been also investigated.
分类： 化学 >> 物理化学 提交时间： 2017-11-05 合作期刊: 《结构化学》
摘要： Using 4΄-methoxy-5-hydroxyisoflavone and 4΄,5-dihydroxy-7-methoxyisoflavone as leding compounds, 6-methoxy-2H-phenanthro[9,10-c]pyrazol-11-ol (1a) and 9-methoxy-2H-phenanthro [9,10-c]pyrazol-6,11-diol (1b) were synthesized by two dehydration processes in the EtOH solution. They were characterized by IR, 1H NMR and 13C NMR. The black prism crystal of 1a was grown by the slow solvent evaporation technique from 40:1 (v/v) CHCl3/MeOH, and it was determined by single-crystal X-ray diffraction. In the crystal structure, 1a was stabilized by intramolecular (O–H···N) and intermolecular (N–H···O, O–H···O, π···π, C–H···π) interactions. In addition, the fluorescence properties of 1a and 1b in the base and neutral media revealed that they possessed excited state intramolecular proton transfer phenomena (ESIPT).
分类： 化学 >> 物理化学 提交时间： 2017-11-05 合作期刊: 《结构化学》
摘要： One new coordination polymer (CP) [Cd(bct)(bib)]·2(H2O) (1), based on 3,5-bis(4΄-carboxy- phenyl)-1,2,4-triazole (H2bct) and 1,4-bis(imidazol-1-ylmethyl)benzene(bib), has been synthesized by hydrothermal method, and its structure was determined and characterized by single-crystal X-ray diffraction analysis, elemental analysis, IR spectroscopy, and thermogravimetric analysis. The crystal structure of CP 1 is a three-dimensional framework with (3,5)-connected topology, where the bct ligands adopt a µ3-kO,kO,kO coordination mode connecting with three Cd centers and the bib ligands adopt a µ2-kN,kN coordination mode bridging two Cd ions. It crystallizes in monoclinic system, space group C2/c, with a = 2.6838(4), b = 1.24221(17), c = 1.8011(2) nm, β = 102.939(2)º, V = 5.8522(13) nm3, Z = 8, C30H27CdN7O6, Mr = 694.00, Dc = 1.566 g/cm3, F(000) = 2784, S = 1.055, R = 0.0294 and wR = 0.0731. Furthermore, solid-state photoluminescence measurements show that CP 1 produces strong emissions at room temperature.
分类： 物理学 >> 核物理学 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： Bio-nanosensors (Bio-NSs) have attracted much attention recently due to their unique properties. Among all of the bio-NSs, the intracellular proton sensor is significant for biomedicine studies and clinic diagnosis. Proton nanosensors (PNSs) with different pH sensitive ranges could satisfy different research requirements. Here we report a facile method to build a PNS with a neutral to basic pH sensitive range, in which the commercial pH indicator, fluoresceinamine (FA), was covalently coupled to the carboxylic-rich amphiphilic polymer (AP) coated gold nanoparticles (AuNPs).
分类： 核科学技术 >> 核材料与工艺技术 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： Understanding uranium-protein interaction is important for revealing the mechanism of uranyl ion (UO22+) toxicity. In this study, we investigated the interaction between UO22+ and a quadruple mutant of cytochrome b5 (E44/48/56A/D60A cyt b5, namely 4A cyt b5) by spectroscopic approaches. The four mutated negatively-charged surface residues of cyt b5 have been considered to be the interactive sites with cytochrome c (cyt c). Also, we studied the interaction between UO22+ and the protein-protein complex of 4A cyt b5-cyt c. The results were compared to the interaction between UO22+ and cyt b5, and the interaction between cyt c and cyt b5-cyt c complex, from previous studies. It was found that the interaction of UO22+-cyt b5, i.e., uranyl ion binding to cyt b5 surface at Glu37 and Glu43 as previously proposed by molecular modeling, is regulated by both surface mutations of cyt b5 and its interacting protein partner cyt c. These provide valuable information on metal-protein-protein interactions and clues for understanding the mechanism of uranyl toxicity.
分类： 物理学 >> 核物理学 提交时间： 2016-09-13
DING Xue-Feng
WEN Liang-Jian
ZHOU Xiang
DING Ya-Yun YE Xing-Chen
ZHOU Li2 LIU Meng-Chao
CAI Hao
CAO Jun
摘要： The fluorescence quantum yield of bis-MSB, a widely used liquid scintillator wavelength shifter, was measured to study the photon absorption and re-emission processes in liquid scintillator. The re-emission process affects the photoelectron yield and distribution, especially in a large liquid scintillator detector, thus must be understood to optimize the liquid scintillator for good energy resolution and to precisely simulate the detector with Monte Carlo. In this study, solutions of different bis-MSB concentration were prepared for absorption and fluorescence emission measurements to cover a broad range of wavelengths. Harmane was used as a standard reference to obtain the absolution fluorescence quantum yield. For the first time we measured the fluorescence quantum yield of bis-MSB up to 430 nm as inputs required by Monte Carlo simulation, which is 0.926±0.053 at λex = 350 nm.
分类： 生物学 >> 生物物理学 提交时间： 2016-05-18
摘要： Herein, a nontoxic nanocomposite is synthesized by reduction of silver nitrate in the presence of a cationic polymer displaying strong antimicrobial activity against bacterial infection. These nanocomposites with a large concentration of positive charge promote their adsorption to bacterial membranes through electrostatic interaction. Moreover, the synthesized nanocomposites with polyvalent and synergistic antimicrobial effects can effectively kill both Gram-positive and Gram-negative bacteria without the emergence of bacterial resistance. Morphological changes obtained by transmission electron microscope observation show that these nanocomposites can cause leakage and chaos of intracellular contents. Analysis of the antimicrobial mechanism confirms that the lethal action of nanocomposites against the bacteria started with disruption of the bacterial membrane, subsequent cellular internalization of the nanopartides, and inhibition of intracellular enzymatic activity. This novel antimicrobial material with good cytocompatibility promotes healing of infected wounds in diabetic rats, and has a promising future in the treatment of other infectious diseases.
分类： 物理学 >> 核物理学 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： Fluorescence spectroscopy was employed to investigate the interaction between fluorophore fluoresceinamine (FA) and bovine serum albumin (BSA) under physiological conditions. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by FA is a result of the formation of a BSA-FA complex. Fluorescence quenching constants were determined using the modified Stern-Volmer equation to provide a measure of the binding affinity between FA and BSA. The results of the thermodynamic parameters G, H, and S at different temperatures indicated that several kinds of interactions, except for the electrostatic interactions play cooperative roles in BSA-FA association. Furthermore, the conformation of BSA upon interaction with FA was also studied by synchrotron fluorescence spectroscopy.
分类： 生物学 >> 生物物理学 >> 生物物理、生物化学与分子生物学 提交时间： 2016-05-11
He, Tao
Gao, Jianmin
Roberts, Mary F.
Gershenson, Anne
Eyles, Stephen J.
Lee, Yan-Jiun
Liu, Wenshe R.
Wang, Jiangyun
摘要： Cation-pi interactions, where protein aromatic residues supply pi systems while a positive-charged portion of phospholipid head groups are the cations, have been suggested as important binding modes for peripheral membrane proteins. However, aromatic amino acids can also insert into membranes and hydrophobically interact with lipid tails. Heretofore there has been no facile way to differentiate these two types of interactions. We show that specific incorporation of fluorinated amino acids into proteins can experimentally distinguish cation-pi interactions from membrane insertion of the aromatic side chains. Fluorinated aromatic amino acids destabilize the cation-pi interactions by altering electrostatics of the aromatic ring, whereas their increased hydrophobicity enhances membrane insertion. Incorporation of pentafluorophenylalanine or difluorotyrosine into a Staphylococcus aureus phosphatidylinositol-specific phospholipase C variant engineered to contain a specific PC-binding site demonstrates the effectiveness of this methodology. Applying this methodology to the plethora of tyrosine residues in Bacillus thuringiensis phosphatidylinositol-specific phospholipase C definitively identifies those involved in cation-pi interactions with phosphatidylcholine. This powerful method can easily be used to determine the roles of aromatic residues in other peripheral membrane proteins and in integral membrane proteins.
分类： 生物学 >> 植物学 提交时间： 2019-03-28 合作期刊: 《干旱区科学》
摘要： The adaption of photosynthesis, being a key metabolic process, plays an important role in plant resistance to air drought. In this study, the Siberian crabapple (Malus baccata L. Borkh.) in the forest-steppe zone of Transbaikalia region, Russia, was subjected to air drought stress and its photosynthesis characteristics were analyzed. The results show that air drought and sufficient soil moisture supply lead to the decrease in the total chlorophyll (Chl) content, while the ratio of Chls to carotenoids is constant in the Siberian crabapple tree. The function of photosystem II (PS-II) in the crabapple trees is characterized by a decrease in the fraction of absorbed light energy spent on the photochemical work and an increase in the proportion of non-photosynthetic thermal quenching. These changes indicate the photosynthetic down-regulation that acts as a universal photoprotective mechanism. During the midday hours, the combination of high air temperature and low air humidity leads to the decrease in the maximum photochemical quantum yield of photosystem II (Fv/Fm) and the efficiency of photosynthesis (PABS). The parameters of leaf gas exchange show the significant differences in these values between the control and experimental variants. During the morning hours, the Siberian crabapple, growing in the Irkutsk City, assimilates carbon dioxide more intensively. Due to the higher air humidity, the stomata are kept open and the necessary amount of carbon dioxide entries the sites of carboxylation. The low air humidity combined with wind in the experimental variants leads to the unreasonably high water loss in the crabapple leaves by more than 27% as compared to the control variant (Irkutsk City). However, water use efficiency in the morning hours increases during plant photosynthetic processes, i.e., 42% higher than that of control. This, apparently, is a reflection of the adaptation processes of the Siberian crabapple to the air drought and parching wind.
分类： 化学 >> 物理化学 提交时间： 2017-11-05 合作期刊: 《结构化学》
摘要： A coordination polymer [Mn2(ctpt)2(aic)2]n (1, ctpt = 2-(4-chloro-phenyl)-1H-1,3,7,8- tetraaza-cyclopenta[l]phenanthrene, H2aic = 5-amino-isophthalic acid) was hydrothermally designed and synthesized. The complex was characterized by elemental analysis, IR spectroscopy, single-crystal X-ray diffraction, and thermogravimetric analysis (TGA). Each Mn(II) atom is linked by the aic ligands with neighbor Mn(II) atoms, forming an infinite one-dimensional (1D) double-chain structure. Complex 1 crystallizes in monoclinic, space group C2/c, with a = 18.23(1), b = 17.27(1), c = 16.69(1) Å, V = 4814.0(7) Å3, C27H16ClMnN5O4, Mr = 564.84, Dc = 1.559 g/cm3, μ(MoKα) = 0.706 mm-1, F(000) = 2296, Z = 8, the final R = 0.0487 and wR = 0.1269 (I > 2σ(I)). The 1D chain structure of complex 1 is stable below 458 ℃. In addition, to elucidate the essential electronic characters of this complex, theoretical calculation analysis of 1 was performed by the PBE0/LANL2DZ method in Gaussian 03 Program.
分类： 物理学 >> 核物理学 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： The contents and distributions of metal elements in the brain are closely related to neurodegenerative diseases. In this study, we examined Fe, Cu and Zn contents in the brain section associated with Parkinson's disease (PD) using synchrotron radiation X-ray fluorescence (SRXRF). PD mouse model induced by 1-methyl-4-phenyl-1,2,3,6-terahydropyridine (MPTP) was used for the elemental analysis (e.g., Fe, Cu and Zn) in the substantia nigra pars compacta (SNpc) region of mice brain tissue samples. We found that mice in the MPTP group had higher contents of Fe, Cu and Zn in the SNpc than the control group. After treating the PD mice with rapamycin, the contents of Fe, Cu and Zn were reduced, the dopamine neurons and motor function were rescued correspondingly. The results prompted that the SRXRF provided an ideal method for tracing and analyzing the metal elements in the brain section to assess the pathological changes of PD model and the therapeutic effect of drugs.
分类： 生物学 >> 生态学 提交时间： 2023-06-13 合作期刊: 《干旱区科学》
摘要：Drought is a critical limiting factor affecting the growth and development of plants in arid and semi-arid areas. Photosynthesis, one of the most important physiological processes of plants, can be significantly inhibited by drought. Photosystem II (PSII) is considered the main attack target when photosynthesis is affected by drought. To clarify how PSII components of the ephemeral plant Erodium oxyrhinchum (grown in the Gurbantunggut Desert, China) respond to drought treatment, we evaluated the functional activity of PSII by determining chlorophyll fluorescence and gas exchange parameters under different drought treatment levels (control (400 mL), moderate drought (200 mL), and severe drought (100 mL)). Under moderate drought treatment, significant decreases were found in net photosynthetic rate (Pn), effective quantum yield of PSII (Y(II)), relative electron transfer rate of PSII (rETR(II)), oxygen-releasing complex, probability of an absorbed exciton moving an electron into the electron transport chain beyond primary quinone receptor QA– (Φ(Eo)), probability of a trapped exciton moving an electron into the electron transport chain beyond primary quinone receptor QA– (ψ(Eo)), and performance index of PSII (PIabs). Compared to control treatment, marked increases were observed in water use efficiency (WUE), relative variable fluorescence at the J step (VJ), initial fluorescence (Fo), and dissipated energy per active reaction center (DIo/RC) under moderate drought treatment, but there were no substantial changes in semi-saturated light intensity (IK), active reaction centers per cross-section (RC/CS), and total performance index of PSII and PSI (PItotal, where PSI is the photosystem I). The changes of the above parameters under severe drought treatment were more significant than those under moderate drought treatment. In addition, severe drought treatment significantly increased the absorbed energy per active reaction center (ABS/RC) and trapping energy per active reaction center (TRo/RC) but decreased the energy transmission connectivity of PSII components, RC/CS, and PItotal, compared to moderate drought and control treatments. Principle component analysis (PCA) revealed similar information according to the grouping of parameters. Moderate drought treatment was obviously characterized by RC/CS parameter, and the values of Fo, VJ, ABS/RC, DIo/RC, and TRo/RC showed specific reactions to severe drought treatment. These results demonstrated that moderate drought treatment reduced the photochemical activity of PSII to a certain extent but E. oxyrhinchum still showed strong adaptation against drought treatment, while severe drought treatment seriously damaged the structure of PSII. The results of this study are useful for further understanding the adaptations of ephemeral plants to different water conditions and can provide a reference for the selection of relevant parameters for photosynthesis measurements of large samples in the field.
分类： 物理学 >> 普通物理:统计和量子力学,量子信息等 提交时间： 2017-05-02
Ouyang, XH [Ouyang, Xinhua][ 1 ]
Li, XL [Li, Xiang-Long][ 2 ]
Bai, YQ [Bai, Yongqi][ 1 ]
Mi, DB [Mi, Dongbo][ 1 ]
Ge, ZY [Ge, Ziyi][ 1 ]
Su, SJ [Su, Shi-Jian][ 2 ]
摘要： An improved concentration of phosphorescent dopant for highly-efficient hybrid white organic light- emitting diodes based on a high radiative exciton ratio (80%) deep-blue emitter has been developed. The high radiative exciton ratio for the deep-blue emitter was found to be the transfer from the higher triplet (T5) to the lowest singlet state (S1) by a “hot-exciton” process. Notably, when the concentration of Ir(2- phq)3 is up to 0.9 wt%, the OLED still exhibited white emission with a maximum total EQE, CE and PE of 22.3%, 53.7 cd A 1 and 60.2 lm W 1, respectively. The exciton transfer mechanism in a high concentration of phosphorescent dopant was also discussed. The studies provide a way to obtain high performance F/P hybrid WOLEDs with a simple architecture and improved doping concentration.
分类： 物理学 >> 普通物理:统计和量子力学,量子信息等 提交时间： 2017-04-06
摘要： An improved concentration of phosphorescent dopant for highly-efficient hybrid white organic light-emitting diodes based on a high radiative exciton ratio (80%) deep-blue emitter has been developed. The high radiative exciton ratio for the deep-blue emitt
分类： 生物学 >> 生物物理学 提交时间： 2016-05-15
摘要： Protein nanocages (PNCs) have been recognized as a promising platform for nanomedicine innovation. Real-time in vivo tracking of PNCs can provide critically important information for the development of PNC-based diagnostics and therapeutics. Here we demonstrate a general strategy for monitoring the behaviors of PNCs in vivo by encapsulating a Ag2S quantum dot (QD) with fluorescence in the second near-infrared window (NIR-II, 1000-1700 nm) inside the PNC, using simian virus 40 (SV40) PNC (PNCSV40) as a model. Benefiting from the high spatiotemporal resolution and deep tissue penetration of NIR-II fluorescence imaging, the dynamic distribution of the PNCSV40 in living mice was tracked in real time with high fidelity, and adopting the PEGylation strategy, surface chemistry-dependent in vivo behaviors of PNCSV40 were clearly revealed. This study represents the first evidence of real-time tracking of the intrinsic behaviors of PNCs in vivo without interference in PNC-host interactions by encapsulating nanoprobes inside. The as-described imaging strategy will facilitate the study of interactions between exogenously introduced PNCs and host body and prompt the development of future protein-based drugs, sensors, and high-efficacy targeted delivery systems.
分类： 核科学技术 >> 粒子加速器 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： Microstructure and oxidation behavior of modified Ni-16Mo-7Cr-4Fe alloys by yttrium microalloying were investigated by scanning electron microscopy, transmission electron microscopy, grazing incident X-ray diffraction and synchrotron radiation X-ray fluorescence. M6C and Ni17Y2 phases were observed and the amount of Ni17Y2 increased with yttrium concentration. When the yttrium concentration increased to 0.43wt.%, some Ni17Y2 chains and multi phase regions containing Ni17Y2, M6C and phase appeared, which is harmful for the oxidation resistance. The alloy containing 0.05wt.% yttrium showed the best oxidation resistance, which derives its oxidation resistance from the adequate concentration of yttrium in the solid-solution ( phase), the formation of the protective layer of YCrO3 and chromia oxide and the strengthening effect of yttrium on oxide boundaries.
分类： 物理学 >> 核物理学 提交时间： 2025-01-20
Yang, Dr. Shumin
Guo, Dr. Ling-Ling
Nan, Dr. Bing
Zhao, Dr. Ying
Wu, Dr. Yan-Qing
Guo, Prof. Zhi
Tian, Dr. Chen
Zhao, Dr. Bo
Xue, Dr. Chao-Fan
Zhao, Dr. Jun
Song, Miss Shuang
Liang, Mr. Zhen-Ye
Li, Prof. Li-Na
Wang, Prof. Yong
Tai, Prof. Renzhong
摘要： The tender energy spectroscopy beamline (BL16U1) is one of the phase-II beamline at the Shanghai Synchrotron Radiation Facility (SSRF). The design and performance of the tender energy spectroscopy beamline at SSRF are described in this paper. Based on a 26 mm-period in vacuum undulator (IVU) source, the beamline is to give an operable energy range between 2.1 and 16 keV, covering the K-edges of those elements from P to Rb and the L3-edges of those elements from Zr to Bi. The principal optical elements of the beamline consist of a toroidal mirror, a liquid-nitrogen cooled double-crystal monochromator, a high harmonic rejection mirror and two pairs of Kirkpatrick–Baez (KB) mirrors. Three end-stations, including the non-focusing, microprobe and sub-microprobe end-stations, are installed on the beamline. X-ray fluorescence (XRF), X-ray absorption spectroscopy (XAS) including X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine-structure (EXAFS), have been achieved under vacuum or He atmosphere at the non-focusing end-station with a spot size of ~ 670×710 μm2. Based on two KB mirrors systems, micro-X-ray fluorescence (μXRF) mapping studies and micro-X-ray absorption near-edge structure (μXANES) will be operated with a spot size of nearly ~ 3.3×1.3 μm2 at the microprobe end-station, and with a smaller spot size of ~ 0.5×0.25 μm2 at the sub-microprobe end-station. Up to now, the non-focusing end-station of the BL16U1 is officially opened to users in Jan. 2024. The microprobe and sub-microprobe end-stations will be opened to users in the near future. This paper describes the characteristics, short-term technical developments and a few of the early experimental results of this new beamline.
分类： 物理学 >> 核物理学 提交时间： 2024-12-12
Yang, Dr. Shumin
Guo, Dr. Ling-Ling
Nan, Dr. Bing
Zhao, Dr. Ying
Wu, Dr. Yan-Qing
Guo, Prof. Zhi
Tian, Dr. Chen
Zhao, Dr. Bo
Xue, Dr. Chao-Fan
Zhao, Dr. Jun
Song, Miss Shuang
Liang, Mr. Zhen-Ye
Li, Prof. Li-Na
Wang, Prof. Yong
Tai, Prof. Renzhong
摘要： The tender energy spectroscopy beamline (BL16U1) is a phase-II beamline project at the Shanghai Synchrotron Radiation Facility (SSRF). The design and performance of the tender energy spectroscopy beamline at SSRF are described in this paper. Based on a 26 mm-period in vacuum undulator (IVU) source, the beamline is to give an operable energy range between 2.1 and 16 keV, covering the K-edges of those elements from P to Rb and the L3-edges of those elements from Zr to Bi. The principal optical elements of the beamline consist of a toroidal mirror, a liquid-nitrogen cooled double-crystal monochromator, a high harmonic rejection mirror and two pairs of Kirkpatrick–Baez (KB) mirrors. Three end-stations, including the non-focusing, microprobe and sub-microprobe end-stations, are installed on the beamline. X-ray fluorescence (XRF), X-ray absorption spectroscopy (XAS) including X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine-structure (EXAFS), have been achieved under vacuum or He atmosphere at the non-focusing end-station with a spot size of ~ 670×710 μm2. Based on two KB mirrors systems, micro-X-ray fluorescence (μXRF) mapping studies and micro-X-ray absorption near-edge structure (μXANES) will be operated with a spot size of nearly ~ 3.3×1.3 μm2 at the microprobe end-station, and with a smaller spot size of ~ 0.5×0.25 μm2 at the sub-microprobe end-station. Up to now, the non-focusing end-station of the BL16U1 is officially opened to users in Jan. 2024. The microprobe and sub-microprobe end-stations will open to users in the near future. This paper describes the characteristics, short-term technical developments and a few of the early experimental results of this new beamline.
点击量
待评议
点击量
下载量
评论
通过
