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马铃薯低氮肥胁迫响应TCP 转录因子的鉴定与分析

Identification and analysis of TCP transcription factors in Solanum tuberosum response to low nitrogen fertilizer stress

摘要:TCP转录因子是植物特有的一类转录因子,参与植物生物学过程的多个方面。为研究马铃薯 TCP转录因子在响应低氮肥胁迫中的作用,该文以氮肥供应不足(0.05 mmolL-1)和氮肥供应充足 (7.5 mmolL-1)条件下的马铃薯根和叶片构建了4个转录组学文库进行测序,并对差异表达的TCP转 录因子进行分析。结果表明:(1)4个转录组文库共鉴定TCP转录因子24个,主要分布在2号、3号和6 号染色体上。(2)结构域分析表明,24个TCP 转录因子均具有典型的basic-Helix-Loop-Helix结构域。 (3)系统进化分析表明,马铃薯与拟南芥TCP蛋白可聚集于一起,分属于10个亚类。(4)转录组测序 结果表明,在低氮肥胁迫下,大多数TCP转录因子被抑制表达,有3个TCP转录因子在根中显著性差异 表达,5个TCP转录因子在叶中特异性表达。(5)根据GO功能分析和马铃薯TCP转录因子与拟南芥TCP 转录因子的亲缘关系分析,推测这些TCP转录因子参与了马铃薯对低氮肥胁迫的响应。该文为进一步 研究马铃薯与其他粮食作物TCP转录因子响应低氮肥胁迫的分子功能奠定了基础。

英文摘要:TCP transcription factors are a type of plant-specific transcription factors, which have been implicated in multiple aspects of plant biological processes. To study the role of potato TCP transcription factors in response to low nitrogen fertilizer stress, here, four mRNA libraries from potato roots and leaves under the conditions of low nitrogen fertilizer (0.05 mmol稬-1) and sufficient nitrogen fertilizer (7.5 mmol稬-1) were constructed for transcriptome sequencing, respectively. Meanwhile, the differentially expressed TCP transcription factor were analyzed. The results were as follows: (1) A total of 24 TCP transcription factors were identified in the four transcriptome libraries, and mainly distributed on the second, third and sixth chromosomes. (2) The analysis of domains showed that all of 24 TCP transcription factors contained basic- Helix-Loop-Helix domain. (3) The phylogenetic analysis showed that the TCP proteins of potato and Arabidopsis thaliana were closely related, and clustered into 10 subgroups. (4) Transcriptome sequencing results showed that the expression levels of most potato TCP transcription factors were suppressed by low nitrogen fertilizer stress. Among them, three TCP transcription factors were significantly differentially expressed in roots, while five TCP transcription factors were specifically expressed in leaves. (5) According to the GO functional analysis and the relationship between the potato and the A. thaliana TCP transcription factors, it is predicted that these TCP transcription factors are involved in response of potato to low-nitrogen fertilizer stress. The research provided a foundation for further study on the molecular role of transcription factors response to low nitrogen fertilizer stress in potato and other crops.

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[V1] 2022-08-05 16:45:06 chinaXiv:202208.00039V1 下载全文
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