Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-07 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the differential expressions of nucleolin in invasive cervical squamous cell carcinoma, cervical intraepithelial neoplasms (CIN) and normal cervical epithelial tissues and explore the role of nucleolin in the carcinogenesis and progression of cervical squamous cell carcinoma. Methods Fifty specimens of invasive cervical squamous cell carcinoma, 65 specimens of CIN, and 60 adjacent normal cervical epithelial tissue specimens were examined immunohistochemically for nucleolin expression. The correlation of nucleolin expression levels with histological grades of invasive cervical squamous cell carcinoma and CIN were analyzed. Results The specimens of invasive cervical squamous cell carcinoma showed a significantly higher positivity rate for nucleolin expression than CIN and normal cervical epithelial tissues, and the rate in CIN tissues was significantly higher than that in normal cervical epithelial tissues (P<0.01). The expression level of nucleolin was significantly higher in invasive cervical squamous cell carcinoma than in CIN and normal cervical epithelia tissues, and higher in CIN than in normal cervical epithelia tissues, whose immunostaining scores were 7.6�0.3, 6.1�.2, and 3.0�.2, respectively (P<0.01). The mean nucleolin immunostaining score was significantly higher in poorly and moderately differentiated than in highly differentiated cervical squamous cell carcinoma (7.9 vs 7.1, P<0.01), and higher in high grade CIN than in low grade CIN tissues (6.0 vs 4.0, P<0.01). Conclusions Overexpression of nucleolin plays an important role during carcinogenesis of cervical squamous cell carcinoma and is positively correlated with tumor progression of CIN and cervical squamous cell carcinoma.
Subjects: Biology >> Ecology submitted time 2018-03-08 Cooperative journals: 《生态学报》
Abstract：三峡水库的运行调度，使库区支流形成了截然不同的3种河段类型：完全受水库蓄水影响的145m回水段（完全河段），既受蓄水影响又受自然洪汛影响的145-175m回水段（双重河段）以及不受蓄水影响的大于175m的自然河流段（自然河段）。为明确库区蓄水对河流不同河段生境因子的影响程度及差异，对三峡库区36条重要支流的254个河段进行河流生境调查，进行不同河段下生境指标的因子分析，并进一步分析水文情势自然性与不同河段各生境因子的相关关系。结果表明，植被状况对3种不同河段来说均为重要生境因子，但受三峡水库蓄水影响，完全河段植被结构不完整；受库区蓄水影响，完全河段与双重河段及自然河段相比，流速流态状况、表层覆盖物状况、河岸带宽度、湿润率等生境因子有明显改变；水文情势自然性对不同河段生境因子的影响不同。
Subjects: Biology >> Ecology submitted time 2018-01-09 Cooperative journals: 《生态学报》
Abstract：三峡水库建成蓄水后，库区支流因水位调度导致河流生境发生了剧烈的变化，消落带的形成使库区河流具有同自然河流截然不同的河流生境，新形势下库区河流生境评价十分必要。国内外现有的评价指标体系及评价方法不能够很好地适应这种特殊生境状况，急需建立或改进并形成新的评价指标体系和评价方法。基于此，分析了大量国内外河流生境评价方法，根据大型水库影响下的库区河流的生态环境特点，构建了包括水文情势、河流形态和河岸带生境3个方面18个指标的库区河流生境评价指标体系，并利用层次分析法（主观赋权法）和熵值法（客观赋权法）结合组合赋权法计算得到了各指标权重。使用新建立的指标体系和方法，以三峡库区支流东溪河、黄金河、汝溪河为例，进行河流生境质量评价发现，52.6%的样点河流生境质量处于优等或良好等级；42.1%为一般等级；5.3%为较差等级；没有最差等级的样点。结果表明，该评价指标体系适合库区支流河流生境状况的特殊性，得到的评价结果能较直观的反应河流生境状况，且操作便捷，数据易获得，具有较强的科学性和可操作性。
Subjects: Medicine, Pharmacy >> Preclinical Medicine submitted time 2017-12-27 Cooperative journals: 《南方医科大学学报》
Abstract： Objective To investigate the effect of docosahexaenoic acid (DHA) on invasiveness of aflatoxin B1 (AFB1)-induced hepatocellular carcinoma cells in vitro. Methods HepG2.2.15 cells were exposed to different concentrations of AFB1 and DHA plus AFB1. The cell migration and invasion were assessed using wound-healing and Transwell assay, and flow cytometry was used to analyze the cell cycle changes. The ultrastructural changes of the cells were observed by transmission electron microscopy. Results Compared with the control group, the cells exposed to2 μmol/L AFB1 showed obviously enhanced migration and invasion with decreased cell ratio in G0/G1 phase and increased cell ratio in G2/M phase but no changes in S phase cells; transmission electron microscopy revealed the presence of multiple nucleoli and significantly increased mitochondria and Golgi apparatus in the exposed cells. Compared with AFB1-exposed cells, the cells treated with DHA and AFB1 showed decreased migration and invasion abilities, and the G0/G1 phase cells increased and G2/M phase cells decreased significantly; ultrastructurally, the cells contained single nucleoli with decreased mitochondria and vacuolization occurred in the cytoplasm. Conclusion DHA can significantly inhibit AFB1-induced enhancement of cell migration and invasion in hepatocellular carcinoma cells in vitro.
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