分类： 核科学技术 >> 辐射物理与技术 提交时间： 2024-04-25
摘要： A Laue microdiffraction beamline (BL03HB) was constructed at the Shanghai Synchrotron Radiation Facility (SSRF). This beamline features two consecutive focusing points in two different sectors within its end station, the first dedicated to protein crystallography and the other tailored to materials science applications. Based on a superbend dipole magnet with a magnetic field of 2.29 T, a two-stage focusing design was implemented with two sets of Kirkpatrick–Baez mirrors to achieve a micro white beam as small as 4.2×4.3 µm 2 at the first sector and 0.9×1.3 µm 2 at the second sector in the standard beamline operation mode at SSRF. The X-ray microbeam in the two sectors can be easily switched between monochromatic and white beams by moving a four-bounce monochromator in or out of the light path, respectively. In the protein crystallography sector, white-beam Laue microdiffraction was demonstrated to successfully determine the structure of protein crystals from only a few images of diffraction data collected by a Pilatus 2M area detector. In the materials science sector, the white-beam Laue diffraction was collected in a reflection geometry using another Pilatus 2M area detector, which could map the microstructural distribution on the sample surface by scanning the samples. In general,the BL03HB beamline promotes the application of Laue microdiffraction in both protein crystallography and materials science. This paper presents a comprehensive overview of the BL03HB beamline, end station, and the first commission results.
分类： 物理学 >> 核物理学 提交时间： 2023-11-22
Qin Xu
Hua-Ting Kong
Ke Liu
Huan Zhou
Kun-Hao Zhang
Wei-Wei Wang
Min-Jun Li
Qiang-Yan Pan
Xing-Ya Wang
Yu-Zhu Wang
Feng Yu
Xing-Yu Gao
Qi-Sheng Wang
摘要： BL10U2 is an undulator-based macromolecular crystallography (MX) beamline located at the 3.5-GeV Shanghai Synchrotron Radiation Facility. BL10U2 is specifically designed for conducting routine and bio safety level-2 (BSL-2) MX experiments utilizing high-flux tunable X-rays with energies from 7 to 18 keV, providing a beam spot size of 20 m (horizontal) 10 m (vertical) at the sample point. Certification by the Shanghai Pudong Municipal Health Commission confirmed the capability to perform BSL-2 MX experiments. The beamline is currently equipped with an Eiger X 16M detector and two newly developed in-house high-precision diffractometers that can be switched to perform conventional or in situ crystal diffraction experiments. An automatic sample changer developed in-house allows fast sample exchange in less than 30s, supporting high-throughput MX experimentation and rapid crystal screening. Data collection from both the diffractometer and detector was controlled by an in-house developed data collection software (Finback) with a user-friendly interface for convenient operation. This study presents a comprehensive overview of the facilities, experimental methods, and performance characteristics of the BL10U2 beamline.
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