Subjects: Biology >> Botany >> Applied botany submitted time 2023-09-20 Cooperative journals: 《广西植物》
Abstract： In order to identify the pathogens that caused tuber rot in Bletilla striata and study the inhibiting effects of herbal extracts on pathogens, the pathogens that caused tuber rot of Bletilla striata were isolated using usual tissue isolation. Morphological and molecular biological techniques were used to identify the strains. And seven herbal extracts were used to study the inhibiting effects on the pathogen. The results were as follows:（1） A total of 14 fungi and 4 bacteria were isolated from diseased leaves, leaf sheaths and tubers. But only strain GF-1 caused disease, whose symptoms consistent with those in the field. The incidences of GF-1 disease reinoculated in the field and laboratory were 100％, respectively. （2）GF-1 was identified as a memmber of Epicoccum, and its colonial morphology is a circular form, with white mycelium, prostrate on the medium, aerial, diaphragms and branches. There are conidia and chlamydospores. （3）At last, the sequence of internal transcribed spacer (ITS) region of GF-1 were analyzed, the length was 522 bp. The sequence was compared with other species in the GenBank and reached 99.62% similarity to E. sorghinum (MN493119.1) isolated form Sorghum, which was closer than others, including E. sorghinum (MF948994.1) isolated form leaves of B. striata. （4）GF-1 could be fully inhibited when the medium contained 0.1- 0.2 g·mL-1 extracts that extracted from 7 Chinese herbal, respectively. And it also could be fully inhibited by 0.05 g·mL-1 of C. cassia or Syringa oblate. In summary, the pathogen that caused tuber rot in Bletilla striata was identified as E. sorghinum. And GF-1 could be fully inhibited cultivated on the medium which contained 0.1- 0.2 g·mL-1 herbal extracts, e.g.: C. cassia, S. oblate, Cyclocarya paliurus, B. striata, Houpoea officinalis, Illicium verum or Cnidium monnieri.
Subjects: Biology >> Botany submitted time 2022-12-29 Cooperative journals: 《广西植物》
LI Jiaqi
LI Yufei
WANG Xiaojing
LI Jianxing
WEN Shujun
HUANG Fuzhao
TAN Yanfang
LU Shuhua
LU Fang
CHEN Ting
GUO Yili
Abstract：
As a repository of genetic information of above-ground vegetation, the soil seed bank plays an important role in the natural succession and renewal of vegetation and the construction of ecological restoration. In this paper, three typical plant communities in the rocky desertification area of Gongcheng Yao Autonomous County were selected for the study, Analysis of soil seed bank structure and diversity in different communities and their response to soil nutrient characteristics, In order to provide a theoretical basis for karst stone desertification management and vegetation restoration in Gongcheng County. The results were as follows：(1) A total of 3 648 seedlings belonging to 55 species in 51 genera and 33 families were detected, These seedlings include 20 species of 1, 2-year-old herbs, 21 species of perennial herbs, 5 species of vines, 3 species of shrubs and 6 species of trees; The average density of soil seed bank of different plant communities in karstic desertification areas of Gongcheng County was: Prunus salicina economic forest (22 493 seeds·m-2) > Cyclobalanopsis glauca secondary forest (1 033 seeds·m-2) > Phyllostachys sulphurea shrub (793 seeds·m-2). (2) As for the plant life type of the soil seed bank, The soil seed bank life type of Prunus salicina economic forest is mainly annual malignant weeds, The plant life type of the soil seed bank in the Cyclobalanopsis glauca secondary forest and Phyllostachys sulphurea shrub is dominated by perennial herbs, while woody plants account for a relatively small proportion; The species diversity and similarity of the soil seed banks of different vegetation types were generally low, while the species composition of the soil seed banks also had low similarity with the above-ground communities. (3) The soil elements in the study area had higher nitrogen content and lower phosphorus content, And phosphorus as a limiting factor for plant growth in Phyllostachys sulphurea shrub and Cyclobalanopsis glauca secondary forest; In a comprehensive view, the plant community in the stone desertification area of Gongcheng County has some native germplasm resources, but overall shows low species diversity, and it is urgent to introduce native tree species artificially to accelerate the positive vegetation succession on the basis of natural sequestration, and pay attention to nutrient management, especially the regulation of phosphorus elements.
Subjects: Biology >> Botany submitted time 2022-09-08 Cooperative journals: 《广西植物》
Abstract： Ardisia humilis is an excellent wild ornamental plant. For the purpose of fully utilization of this elite germplasm, the flowering biology and breeding system of this plant had been expre in this study. We conducted field investigation in the flowering phenology, floral morphology, and floral visitor on Ardisia humilis individuals introduced in homogeneous garden. Furthermore, pollen viability and stigma receptivity were detected by pollen germination in vitro and by benzidine-hydrogen peroxide method, respectively. Combined with artificial pollination,the self-compatibility and the self-pollination of Ardisia humilis were also analyzed in this study. The results were as follows: (1) The florescence of Ardisia humilis was from mid-late May to early June. The flowering span of the population, single plant and single flower were approximately 25 d, 8 d and 1 d, respectively. (2) Ardisia humilis developed protogynic bisexual flowers, with partial overlap on mature stage of pistil and that of stamen, while stigma remained receptivity until 1 d after stamens fall. (3) With a OCI value of 4 and pollen / ovule value of 5.61�103, the breeding system of Ardisia humilis were considered to be outcrossing, with partial self-compatibility, and the pollinator insects were essential. Lasioglossum sp.1, Lasioglossum sp.2 and Halictus sp. were the most observed floral visitors on Ardisia humilis.(4) In artificial pollination, The seed set of natural pollination ranked the highest level (averaged 52.96%), followed by that of autogamy (52.29%) and of artificial cross pollination (50.33%), while that of artificial geitonogamy (28.67%) was the lowest one. This study indicated that, beside of being excellent flowering plant, Ardisia humilis is also a potentially important ornamental fruit plant due to the generalized pollination system and the strongly autogamy ability which enhance the high success of fruits development from blossoms.
Subjects: Biology >> Botany >> Applied botany submitted time 2022-08-05 Cooperative journals: 《广西植物》
Abstract： Inorder to study the effect of soil factors on the the growth and medicinal component of Paris polyphylla var. chinensis, and to provide a support for the artificial cultivation, the biomass and the polyphyllins content of different samples from different origins were determined, and soil composition was measured. Then the correlation between soil factors and the growth ， and medicinal component were analyzed. The results were as follows: (1)There were differences in soil composition in different place, while the yield and polyphyllins content in samples in different place were quite different; (2)Correlation analysis showed that the dry weight was significantly positively correlated with organic matter, total nitrogen, and alkaline nitrogen, the polyphyllin I content was significantly positively related with organic matter and available phosphorus, the polyphyllin Ⅱ content was significantly positively correlated with available phosphorus and available potassium; (3)Multiple linear regression analysis showed that the dry weight was dominantly affected by alkaline nitrogen, the polyphyllin I was dominantly affected by organic matter, the polyphyllins Ⅱ was dominantly affected by available phosphorus, and was linear negatively correlated with alkaline nitrogen. In summary, the main soil factors affecting dry weight was alkaline nitrogen, the main soil factors affecting the polyphyllin total content was organic matter and available phosphorus.
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