Drug consistency evaluation of atorvastatin by urine proteomic

Subjects: Biology >> Biochemistry Subjects: Medicine, Pharmacy >> Pharmacology submitted time 2022-03-09

赵晨阳 陈昱臻 付晓越 侯芙芳 高友鹤

Abstract：

[Objective] The drug consistency evaluation of atorvastatin by urine proteomic analysis.

[Methods] Intragastric administration rat models were established by atorvastatin from two companies. Urine samples were collected from rats before and after intragastric administration. Urine proteins were profiled using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), and the biological pathway of the differential proteins was analyzed by DAVID database.

[Results] The differential proteins were selected by comparing the after intragastric administration to before intragastric administration. A total of 116 differential proteins were identified in group A and 66 differential proteins in group B, and 24 proteins were identified in common between two groups. These differential proteins tend to be involved in biological pathways such as cell adhesion, negative regulation of endopeptidase activity and proteolysis.

 [Conclusions] For two kind of atorvastatin, we can detect small differences in urinary protein between them, which confirms the sensitivity of urinary protein and suggests that urine proteomics has great potential for the Drug consistency evaluation.

Peer Review Status:Awaiting Review

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