Subjects: Biology >> Botany submitted time 2023-08-25 Cooperative journals: 《广西植物》
Abstract： Rosa rugosa is a deciduous shrub belonging to Rosa L. in the Rosaceae family. It has high ornamental value and commercial value, but its single color limits the development and utilization of rose and its application in landscape architecture. In order to explore the coloring substances of three different varieties of roses, ‘Rosa rugosa × Rosa sertata ’, ‘Rosa Crimson Glory’ and ‘Rosa alba’, this study used ultra-high performance liquid chromatography-four-pole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to detect thetypes and contents of flavonoids in petals. The KEGG database was used to enrich the differential metabolites, screen out the key metabolites, and analyze thecorrelation with the phenotypic value of flower color. The results were as follows: (1) A total of 58 metabolites were detected in petals of different rose varieties, of which only one anthocyanin was cyanidin-3-O-glucoside, accounting for 30.45%. (2) K-means clustering analysis showed that a total of 12 key metabolites were annotated to the KEGGmetabolic pathway. Among them, pinocembrin and myricetin were the main substances that determined the red color of ‘Rosa rugosa × Rosa sertata’ and ‘Rosa Crimson Glory’, and eriodictyol, luteolin and kaempferol were the main substances that determined the white color of ‘Rosa alba’. In conclusion, this study can provide a theoretical basis for the breeding of roses with specific colors and promote the application of roses in landscaping.
Subjects: Biology >> Botany submitted time 2023-08-24 Cooperative journals: 《广西植物》
Abstract： Rosa rugosa is a deciduous shrub belonging to Rosa L. in the Rosaceae family. It has high ornamental value and commercial value, but its single color limits the development and utilization of rose and its application in landscape architecture. In order to explore the coloring substances of three different varieties of roses, ‘Rosa rugosa × Rosa sertata ’, ‘Rosa Crimson Glory’ and ‘Rosa alba’, this study used ultra-high performance liquid chromatography-four-pole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to detect thetypes and contents of flavonoids in petals. The KEGG database was used to enrich the differential metabolites, screen out the key metabolites, and analyze thecorrelation with the phenotypic value of flower color. The results were as follows: (1) A total of 58 metabolites were detected in petals of different rose varieties, of which only one anthocyanin was cyanidin-3-O-glucoside, accounting for 30.45%. (2) K-means clustering analysis showed that a total of 12 key metabolites were annotated to the KEGGmetabolic pathway. Among them, pinocembrin and myricetin were the main substances that determined the red color of ‘Rosa rugosa × Rosa sertata’ and ‘Rosa Crimson Glory’, and eriodictyol, luteolin and kaempferol were the main substances that determined the white color of ‘Rosa alba’. In conclusion, this study can provide a theoretical basis for the breeding of roses with specific colors and promote the application of roses in landscaping.
Subjects: Biology >> Botany >> Applied botany submitted time 2023-08-04 Cooperative journals: 《广西植物》
Abstract： Rosa rugosa is a deciduous shrub belonging to Rosa L. in the Rosaceae family. It has high ornamental value and commercial value, but its single color limits the development and utilization of rose and its application in landscape architecture. In order to explore the coloring substances of three different varieties of roses, ‘Rosa rugosa × Rosa sertata ’, ‘Rosa Crimson Glory’ and ‘Rosa alba’, this study used ultra-high performance liquid chromatography-four-pole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) to detect thetypes and contents of flavonoids in petals. The KEGG database was used to enrich the differential metabolites, screen out the key metabolites, and analyze thecorrelation with the phenotypic value of flower color. The results were as follows: (1) A total of 58 metabolites were detected in petals of different rose varieties, of which only one anthocyanin was cyanidin-3-O-glucoside, accounting for 30.45%. (2) K-means clustering analysis showed that a total of 12 key metabolites were annotated to the KEGGmetabolic pathway. Among them, pinocembrin and myricetin were the main substances that determined the red color of ‘Rosa rugosa × Rosa sertata’ and ‘Rosa Crimson Glory’, and eriodictyol, luteolin and kaempferol were the main substances that determined the white color of ‘Rosa alba’. In conclusion, this study can provide a theoretical basis for the breeding of roses with specific colors and promote the application of roses in landscaping.
Subjects: Geosciences >> Geography submitted time 2020-12-17 Cooperative journals: 《干旱区研究》
Abstract：为选择抗旱优良的唐古特白刺家系，本实验于2015、2016年和2018年分别对来自兰州和武威试验点的31个唐古特白刺家系进行可溶性糖（SS）、可溶性蛋白（SP）、游离脯氨酸（Pro）、过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）以及叶绿素（Chl）含量测定，运用主成分分析法和隶属函数法对家系抗旱性进行综合评价选择出抗旱优良家系并进行对比分析。结果表明：运用2种综合评价方法结果重合率在80%以上，以20%入选率选择抗旱优良家系，2试验点的j3-9、jc-8、w3-15、w3-12等4个家系在2015年、2016年均入选，在2018年抗旱性明显优于其他家系，入选家系在不同年份不同试验点的抗旱性表现一致，说明唐古特白刺抗旱优良家系在不同生理阶段不同环境下抗旱性表现稳定，对其进行早期选择具有可靠性。
Subjects: Environmental Sciences, Resource Sciences >> Basic Disciplines of Environmental Science and Technology submitted time 2020-06-21 Cooperative journals: 《干旱区研究》
Abstract：本实验于武威市凉州区育苗建立试验地，分别在2014、2015、2016、2017、2018年7月，以红砂家系（2a、3a、4a、5a、6a生红砂）叶片为试验材料，测定叶片中可溶性蛋白(SP)、可溶性糖(SS)、丙二醛(MDA)、脯氨酸(Pro)、叶绿素a(Chl a)、叶绿素b(Chl b)、总叶绿素(Chl a+b)、叶绿素a/b(Chl a/b)和叶绿素荧光等16个指标，连续5 a研究红砂家系抗旱性早期选择可行性及性状表现稳定性，选择和综合评价抗旱优良红砂家系。结果表明:可溶性蛋白、可溶性糖、脯氨酸及叶绿素荧光相关指标在77个红砂家系间存在较大的差异，为抗旱优良红砂家系选择创造条件；通过5 a连续观察及测定，2 a、3 a、4 a生红砂家系综合排序逐年表现稳定，重合率逐渐增加至83.33%，5 a、6 a生各层次红砂家系表现稳定，重合率大于83.33%；经过综合分析筛选出小甘沟-3号(XGG-3)、巴彦浩特3-4号(BYHT-3)、乌达-1号(WD-1)、泉眼山-2号(QYS-2)、海石湾-1号(HSW-1)等5个优良抗旱家系，入选率为6.49%。
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