分类： 核科学技术 >> 核材料与工艺技术 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： Graphene oxide (GO) nanosheets possess several advantages, such as a large surface, outstanding bio-compatibility, and straightforward chemical modification capability. They also have great potential as a drug-carrier. In this article, we radiolabeled GO nanosheets with 99mTc, which satisfies the potential needs of micro-SPECT imaging probes in pre-clinical and clinical research. GO nanosheets were synthesized through the modified Hummers method, then GO nanosheets with azide group covalently functionalized in two steps were conjugated to DOTA (1,4,7,10-tetraazacyclododecane-N,N,N,N -tetraacetic acid) and functionalized with an alkynyl group by means of click chemistry. Then through the addition and reduction of technetium-99m, the 99mTc-DOTA-GO were attained. DOTA-conjugated GOs with lateral dimensions of 500600 nm were synthesized. Both atomic force microscopy (AFM) and FT-IR were performed to characterize the GO-DOTA. Labeling efficiency of GO-DOTA with 99mTc was >90% and radiochemical purities were >96% with purification. We successfully synthesized graphene oxide derivatives, DOTA-conjugated GOs, via Click Chemistry, and it was labeled with 99mTc for SPECT imaging. High radiolabeling efficiency makes GO nanosheets suitable platforms for future molecular imaging research.
分类： 物理学 >> 核物理学 提交时间： 2023-06-18 合作期刊: 《Nuclear Science and Techniques》
摘要： The contents and distributions of metal elements in the brain are closely related to neurodegenerative diseases. In this study, we examined Fe, Cu and Zn contents in the brain section associated with Parkinson's disease (PD) using synchrotron radiation X-ray fluorescence (SRXRF). PD mouse model induced by 1-methyl-4-phenyl-1,2,3,6-terahydropyridine (MPTP) was used for the elemental analysis (e.g., Fe, Cu and Zn) in the substantia nigra pars compacta (SNpc) region of mice brain tissue samples. We found that mice in the MPTP group had higher contents of Fe, Cu and Zn in the SNpc than the control group. After treating the PD mice with rapamycin, the contents of Fe, Cu and Zn were reduced, the dopamine neurons and motor function were rescued correspondingly. The results prompted that the SRXRF provided an ideal method for tracing and analyzing the metal elements in the brain section to assess the pathological changes of PD model and the therapeutic effect of drugs.
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