Subjects: Biology >> Botany submitted time 2022-12-29 Cooperative journals: 《广西植物》
WU Yong
WEI Aiji
YANG Ku
LI Jingui
DONG Sisi
LIU Dali
HOU Chunlan
LIU Honglin
CHENG Chi
XUE Feilong
LIU Songqing
Abstract：
To investigate the synergistic effect of Rosa roxbunghii Tratt polysaccharide（RRTP）and rosa roxbunghii tratt insoluble dietary fiber（RTIDF）on hypoglycemic function. In this study, RRTP and RTIDF were extracted, isolated and purified, the antioxidant and hypoglycemic activities of polysaccharides were measured by in vitro experiment and the relationship between hypoglycemic function and intestinal microbiota structure in mice was analyzed in vivo intervention experiment of type Ⅱ diabetes in mice. The results showed that: (1) RRTP had good free radical scavenging ability in vitro, and could significantly inhibit α-glucosidase and α-amylase activities with IC50 of 0.293 and 4.251 mg·mL-1, respectively. RTIDF only showed certain inhibitory activity on α-amylase activity. (2) After the intervention of the extractive in the model mice, the tendency of the obese mice to continue to lose weight was reversed. Compared with CK group, the blood glucose level of RTIDF and RRTP+RTIDF mice was significantly down-regulated, and the activity of CAT enzyme in serum was significantly enhanced. RRTP+RTIDF group was superior to RTIDF group. (3) In addition, the extract intervention could reduce the inflammatory factors in the liver, relieve the degree of cell swelling, increase the number of absorbing cells in the cecum, and restore the intestinal wall mucosal layer. (4) Further analysis of intestinal flora showed that RTIDF and RRTP could reduce the proportion of bacteroidetes to firmicutes, increase the abundance of beneficial bacteria such as acetobacter, but RTIDF had more significant regulation effect on the population. Therefore, based on in vitro hypoglycemic simulation and in vivo intervention results, RRTP and RTIDF have a certain synergistic effect on glucose intervention in diabetic mice, it may be used together as an intervention to improve type II diabetes.
Subjects: Biology >> Botany submitted time 2022-11-24 Cooperative journals: 《广西植物》
WU Yong
WEI Aiji
YANG Kun
LI Jingui
DONG Sisi
LIU Dali
HOU Chunlan
LIU Honglin
CHENG Chi
XUE Feilong
LIU Songqing
Abstract：
To investigate the synergistic effect of Rosa roxbunghii Tratt polysaccharide（RRTP）and rosa roxbunghii tratt insoluble dietary fiber（RTIDF）on hypoglycemic function. In this study, RRTP and RTIDF were extracted, isolated and purified, the antioxidant and hypoglycemic activities of polysaccharides were measured by in vitro experiment and the relationship between hypoglycemic function and intestinal microbiota structure in mice was analyzed in vivo intervention experiment of type Ⅱ diabetes in mice. The results showed that: (1) RRTP had good free radical scavenging ability in vitro, and could significantly inhibit α-glucosidase and α-amylase activities with IC50 of 0.293 and 4.251 mg·mL-1, respectively. RTIDF only showed certain inhibitory activity on α-amylase activity. (2) After the intervention of the extractive in the model mice, the tendency of the obese mice to continue to lose weight was reversed. Compared with CK group, the blood glucose level of RTIDF and RRTP+RTIDF mice was significantly down-regulated, and the activity of CAT enzyme in serum was significantly enhanced. RRTP+RTIDF group was superior to RTIDF group. (3) In addition, the extract intervention could reduce the inflammatory factors in the liver, relieve the degree of cell swelling, increase the number of absorbing cells in the cecum, and restore the intestinal wall mucosal layer. (4) Further analysis of intestinal flora showed that RTIDF and RRTP could reduce the proportion of bacteroidetes to firmicutes, increase the abundance of beneficial bacteria such as acetobacter, but RTIDF had more significant regulation effect on the population. Therefore, based on in vitro hypoglycemic simulation and in vivo intervention results, RRTP and RTIDF have a certain synergistic effect on glucose intervention in diabetic mice, it may be used together as an intervention to improve type II diabetes.
Subjects: Geosciences >> Other Disciplines of Geosciences submitted time 2019-09-11 Cooperative journals: 《干旱区研究》
Abstract：丛枝菌根(Arbuscular mycorrhizas，AM)真菌是土壤生态系统中广泛分布的优秀微生物资源，是具有重要经济价值的药用植物。本文基于人工培育条件下对中药材红花(Carthamus tinctorius)分别进行摩西球囊霉(Glomus mosseae)、根内球囊霉(Glomus intraradices)和混合菌(G.mosseae，Glomus etunicatum，Glomus microagregatum，Glomus caledonium，Glomus cladoideum和G.intraradices) 3种AM真菌接种处理，并以接种等量灭活菌种作为对照组处理。采用高效液相色谱法测定不同采摘时期红花绒中羟基红花黄色素A和山奈素含量的变化。结果表明：与对照组相比，AM真菌接种处理显著促进了红花生物量的积累，且AM真菌混合接种效果显著高于AM真菌单接种；在次生代谢产物合成方面，AM真菌接种处理以及不同采摘期的红花中羟基红花黄色素A和山奈素的含量差异不显著。表明AM真菌虽能促进红花的生长，但不影响羟基红花黄色素A和山奈素的合成。
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