Complete Genomics refreshes the global gene sequencing throughput record and achieves a new low price

On February 7, 2023, at the AGBT (Advances in Genome Biology and Technology, Genome Biology Technology Progress Conference), Complete Genomics, a subsidiary of MGI, released two new sequencers DNBSEQ-T20×2 and DNBSEQ- G99, and a stLFR kit.

1. As an ultra-high-throughput sequencer, DNBSEQ-T20×2 can complete 50,000 cases of human whole genome sequencing per year, creating a new record for the throughput of a global gene sequencer, and the cost of a single sequence is less than $100, achieving the best The cost of scale has built a "super sequencing factory" with a super production scale for the industry.
2. DNBSEQ-G99, the latest model of the G-series, is an ultra-high-speed gene sequencer that provides low-to-medium range throughput, making it the best choice for targeted gene sequencing and small genome sequencing.
3. stLFR (single tube long fragment reads, single tube long fragment reads), can barcode the DNA of massively parallel sequencing (MPS) and next-generation sequencing (NGS), the combination of stLFR kit and PCR-free WGS can be More complete and accurate haplotype-phased genomes at an affordable price, setting a new WGS standard for research and clinical applications.

During the conference, Complete Genomics also announced that its flagship sequencer, DNBSEQ-T7, will be available at $1.5/Gb, and users can obtain high-quality DNBSEQ genomes for $150.

forward from:

https://www.prnewswire.com/news-releases/complete-genomics-drops-genome-sequencing-price-to-sub-100-at-agbt-general-meeting-301740723.html)

Recommended reading for relevant papers (source ChinaXiv-Global):

http://global.chinaxiv.org/#/browse/browseList?seachword=DESIGNING%20OF%20CUSTOM%20BARCODES%20FOR%20SEQUENCING%20ON%20THE%20MGI%20PLATFORM

DESIGNING OF CUSTOM BARCODES FOR SEQUENCING ON THE MGI PLATFORM

Keywords: genomics

DOI: 10.1101/2022.09.07.506907

Source: biorxiv

Submit Date: 2022-09-08

The recently launched MGI platform (DNBSEQ-G50, -G400 and -T7 sequencers) has been gaining popularity for next-generation sequencing. However, the barcode set for library preparation provided by the manufacturer has certain limitations on the number of samples that can be sequenced at a time and the compatibility of barcodes from different or incomplete sets as well as a ratio between the samples. In this paper, we present a protocol for designing custom barcodes expanding the pre-existing barcode set and demonstrate its performance on the MGI Tech Co. Ltd. (China) machines. We developed a universal "quad method" which allows for simultaneous sequencing of any number of samples up to 252 per lane which is multiple of 4 or 4n+2. Here, we describe this method, its analysis, verification, and integration into the sequencing as well as its validation for sequencing using the DNBSEQ G-400 machine.