摘要: The cotton bollworm, Helicoverpa armigera, has developed strong resistance to many insecticides. Sterol Carrier Protein-2 (SCP-2) is an important non-specific lipid transfer protein in insects and appears to be a potential new target. In order to elucidate the structure and function of Helicoverpa armigera SCP-2 (HaSCP-2), NMR spectroscopy, docking simulations, mutagenesis and bioassays were performed. HaSCP-2 composed of five alpha-helices and four stranded beta-sheets. The folds of alpha-helices and beta-sheets interacted together to form a hydrophobic cavity with putative entrance and exit openings, which served as a tunnel for accommodating and transporting of lipids. Several sterols and fatty acids could interact with HaSCP-2 via important hydrophobic sites, which could be potential targets for insecticides. Mutagenesis experiments indicated Y51, F53, F89, F110, I117 and Q131 may be the key functional sites. HaSCP-2 showed high cholesterol binding activity and SCP-2 inhibitors (SCPIs) could inhibit the biological activity of HaSCP-2. SCPI-treated larvae at young stage showed a significant decrease of cholesterol uptake in vivo. Our study describes for the first time a NMR structure of SCP-2 in lepidopteran H. armigera and reveals its important function in cholesterol uptake, which facilitates the screening of effective insecticides targeting the insect cholesterol metabolism.
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期刊:
SCIENTIFIC REPORTS
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分类:
生物学
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生物物理学
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引用:
ChinaXiv:201605.01463
(或此版本
ChinaXiv:201605.01463V1)
DOI:10.12074/201605.01463V1
CSTR:32003.36.ChinaXiv.201605.01463.V1
- 推荐引用方式:
Ma, Haihao,Ma, Yuemin,Liu, Kaiyu,Peng, Jianxin,Peng, Rong,Liu, Xuehui,Xu, Pingyong,Dyer, David H.,Lan, Que,Hong, Huazhu.(2016).NMR structure and function of Helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm.SCIENTIFIC REPORTS.[ChinaXiv:201605.01463]
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