：Land use/land cover (LULC) change and climate change are two major factors affecting the provision of ecosystem services which are closely related to human well-being. However, a clear understanding of the relationships between these two factors and ecosystem services in Central Asia is still lacking. This study aimed to comprehensively assess ecosystem services in Central Asia and analyze how they are impacted by changes in LULC and climate. The spatiotemporal patterns of three ecosystem services during the period of 20002015, namely the net primary productivity (NPP), water yield, and soil retention, were quantified and mapped by the Carnegie-Ames-Stanford Approach (CASA) model, Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model, and Revised Universal Soil Loss Equation (RUSLE). Scenarios were used to determine the relative importance and combined effect of LULC change and climate change on ecosystem services. Then, the relationships between climate factors (precipitation and temperature) and ecosystem services, as well as between LULC change and ecosystem services, were further discussed. The results showed that the high values of ecosystem services appeared in the southeast of Central Asia. Among the six biomes (alpine forest region (AFR), alpine meadow region (AMR), typical steppe region (TSR), desert steppe region (DSR), desert region (DR), and lake region (LR)), the values of ecosystem services followed the order of AFR>AMR>TSR>DSR> DR>LR. In addition, the values of ecosystem services fluctuated during the period of 20002015, with the most significant decreases observed in the southeast mountainous area and northwest of Central Asia. LULC change had a greater impact on the NPP, while climate change had a stronger influence on the water yield and soil retention. The combined LULC change and climate change exhibited a significant synergistic effect on ecosystem services in most of Central Asia. Moreover, ecosystem services were more strongly and positively correlated with precipitation than with temperature. The greening of desert areas and forest land expansion could improve ecosystem services, but unreasonable development of cropland and urbanization have had an adverse impact on ecosystem services. According to the results, ecological stability in Central Asia can be achieved through the natural vegetation protection, reasonable urbanization, and ecological agriculture development.