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  • Plant growth-promoting properties and anti-fungal activity of endophytic bacterial strains isolated from Thymus altaicus and Salvia deserta in arid lands

    分类: 生物学 >> 植物学 提交时间: 2023-11-13 合作期刊: 《干旱区科学》

    摘要: Endophytes, as crucial components of plant microbial communities, significantly contribute to enhancing the absorption of nutrients such as nitrogen and phosphorus by their hosts, promote plant growth, and degrade pathogenic fungal mycelia. In this study, an experiment was conducted in August 2022 to explore the growth-promoting potential of endophytic bacterial strains isolated from two medical plant species, Thymus altaicus and Salvia deserta, using a series of screening media. Plant samples of Thymus altaicus and Salvia deserta were collected from Zhaosu County and Habahe County in Xinjiang Uygur Autonomous Region, China, in July 2021. Additionally, the inhibitory effects of endophytic bacterial strains on the four pathogenic fungi (Fusarium oxysporum, Fulvia fulva, Alternaria solani, and Valsa mali) were determined through the plate confrontation method. A total of 80 endophytic bacterial strains were isolated from Thymus altaicus, while a total of 60 endophytic bacterial strains were isolated from Salvia deserta. The endophytic bacterial strains from both Thymus altaicus and Salvia deserta exhibited plant growth-promoting properties. Specifically, the strains of Bacillus sp. TR002, Bacillus sp. TR005, Microbacterium sp. TSB5, and Rhodococcus sp. TR013 demonstrated strong cellulase-producing activity, siderophore-producing activity, phosphate solubilization activity, and nitrogen-fixing activity, respectively. Out of 140 endophytic bacterial strains isolated from Thymus altaicus and Salvia deserta, 104 strains displayed anti-fungal activity against Fulvia fulva, Alternaria solani, Fusarium oxysporum, and Valsa mali. Furthermore, the strains of Bacillus sp. TR005, Bacillus sp. TS003, and Bacillus sp. TSB7 exhibited robust inhibition rates against all the four pathogenic fungi. In conclusion, the endophytic bacterial strains from Thymus altaicus and Salvia deserta possess both plant growth-promoting and anti-fungal properties, making them promising candidates for future development as growth-promoting agents and biocontrol tools for plant diseases.

  • An improvement in APOR process I-uranium/plutonium separation process

    分类: 核科学技术 >> 裂变堆工程技术 提交时间: 2023-06-18 合作期刊: 《Nuclear Science and Techniques》

    摘要: The reduction stripping behavior of Pu(IV) from 30%TBP/OK with hydroxysemicarbazide (HSC) was investigated, and the separation efficiency of HSC and DMHAN-MMH for U/Pu partitioning in Purex process was compared. The results show that HSC can effectively realize the separation of Pu from U; using mixer-settlers to simulate U/Pu separation in 1B bank of PUREX, from 16-stage counter current extraction experiment (in which 6 stages for supplemental extraction, 10 stages for stripping) with flow rate ratio (1BF:1BX:1BS) =4:1:1 in 1B contactor, good result was achieved that the yields are both more than 99.99% for uranium and Pu, the separation factor of plutonium from uranium (SFPu/U) is 2.8�104, and separation factor of uranium from plutonium (SFU/Pu) is 5.9�104. As a stripping reductant, HSC can effectively achieve the separation of Pu from U and the separation effect is nearly the same with DMHAN-MMH, which contributed to replace enough the latter with HSC in the U/Pu separation in Advanced Purex Process Based on Organic Reagent (APOR) process.

  • Introduction of the X-ray diffraction beamline of SSRF

    分类: 核科学技术 >> 粒子加速器 提交时间: 2023-06-18 合作期刊: 《Nuclear Science and Techniques》

    摘要: The X-ray diffraction beamline developed at Shanghai Synchrotron Radiation Facility (SSRF)is located at the BL14B1 bending magnet port of the 3.5 GeV storage ring. The beamline optics is based on a collimating mirror, a sagittally focused double crystal monochromator and a focusing mirror. Photon flux of 4.43�1011 phs/s at 10 keV is obtained. The primary instrument equipped in the experimental end-station is a Huber 5021 six-cycle diffractometer. BL14B1 is a general purpose X-ray diffraction beamline and focused on material science, condensed matter physics and other relevant fields looking for structural information.

  • Diversity of cultivable endophytic bacteria associated with halophytes in Xinjiang of China and their plant beneficial traits

    分类: 地球科学 >> 地理学 提交时间: 2021-09-08 合作期刊: 《干旱区科学》

    摘要: Endophytic bacteria from halophytes have a wide range of application prospects in various fields, such as plant growth-promoting, biocontrol activity and stress resistance. The current study aimed to identify cultivable endophytic bacteria associated with halophytes grown in the salt-affected soil in Xinjiang Uygur Autonomous Region, China and to evaluate their plant beneficial traits and enzyme-producing activity. Endophytic bacteria were isolated from Reaumuria soongorica (PalL Maxim.), Artemisia carvifolia (Buch.-Ham. ex Roxb. Hort. Beng.), Peganum harmala L. and Suaeda dendroides (C. A. Mey. Moq.) by using the cultural-dependent method. Then we classified these bacteria based on the difference between their sequences of 16S rRNA (16S ribosomal RNA) gene. Results showed that the isolated bacteria from R. soongorica belonged to the genera Brucella, Bacillus and Variovorax. The bacteria from A. carvifolia belonged to the genera Micromonospora and Brucella. The bacteria from P. harmala belonged to the genera Paramesorhizobium, Bacillus and Peribacillus. The bacteria from S. dendroides belonged to the genus Bacillus. Notably, the genus Bacillus was detected in the three above plants, indicating that Bacillus is a common taxon of endophytic bacteria in halophytes. And, our results found that about 37.50% of the tested strains showed strong protease-producing activity, 6.25% of the tested strains showed strong cellulase-producing activity and 12.50% of the tested strains showed moderate lipase-producing activity. Besides, all isolated strains were positive for IAA (3-Indoleacetic acid) production, 31.25% of isolated strains exhibited a moderate phosphate solubilization activity and 50.00% of isolated strains exhibited a weak siderophore production activity. Our findings suggest that halophytes are valuable resources for identifying microbes with the ability to increase host plant growth and health in salt-affected soils.

  • Correlation Analysis of Transient Heat Transfer Characteristics for Air Pre- cooling Aggregate

    分类: 动力与电气工程 >> 工程热物理学 提交时间: 2017-03-31 合作期刊: 《热科学学报》

    摘要: In dam works, air precooling of aggregate is a common and effective method to avoid temperature cracks in con- crete structure. In order to offer a reliable design theory for the air precooling process to avoid unreasonable energy consumption, the transient heat transfer characteristics of the aggregate are intensively analyzed. The combined structure of the aggregate and the interstitial space in the hopper is treated as a porous structure, and the space-average method is used to simulate the transient heat transfer process. Simulation results show that size of the hopper and the average air velocity in the cross section have great influence on the transient heat transfer process of the aggregate, while the porosity in the range of 0.4‒0.5 has little influence. Nomograms are abstracted from simulation results, and then correlations of the compared excess temperature are precisely fitted to predict the air precooling transient heat transfer process of the aggregate.